Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization

Propolis is a resinous vegetal exudate modified by bees, and is interesting as a preservative and potentially functional product. This work dealt with studying the common phenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas. Both hyaluronidase and angiote...

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Bibliographic Details
Published in:Antioxidants 2020-01, Vol.9 (1), p.75
Main Authors: Osés, Sandra M, Marcos, Patricia, Azofra, Patricia, de Pablo, Ana, Fernández-Muíño, Miguel Ángel, Sancho, M Teresa
Format: Article
Language:English
Subjects:
ace inhibitory activity
Acids
Angiotensin
antioxidant capacities
Antioxidants
Caffeic acid
Calibration
Catechin
Coumaric acid
Ethanol
Ferulic acid
Flavonoids
Free radicals
High-performance liquid chromatography
hyaluronidase inhibitory activity
Hydroxyl radicals
Inflammation
p-Coumaric acid
Peptidyl-dipeptidase A
Phenolic compounds
phenolic profile
Physiology
Preservatives
Propolis
Raw materials
Resins
Risk factors
Superoxide anions
Uric acid
Vitamin E
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Summary:Propolis is a resinous vegetal exudate modified by bees, and is interesting as a preservative and potentially functional product. This work dealt with studying the common phenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas. Both hyaluronidase and angiotensin converting enzyme (ACE) inhibitory activities were also assessed and related when possible with particular phenolic compounds. High performance liquid chromatography-ultraviolet detection (HPLC-UV) analysis showed that every propolis contained -coumaric acid (1.2-12.2 mg/g) and ferulic acid (0.3-11.0 mg/g). Pinocembrin, catechin, and caffeic acid phenethyl ester (CAPE) plus galangin were the main flavonoids. Antioxidant activities were higher than 280 µmol trolox/g for trolox equivalent antioxidant capacity (TEAC), 0.099 mmol uric acid/g for radical-scavenging effect on hydroxyl radicals, and 0.19 mg/mL for half maximal inhibitory concentration (IC ) of antioxidant activity against superoxide anion radical. Working with solutions of 10 mg/mL propolis, hyaluronidase inhibitory activity ranged between 0% and 68.20%, being correlated to ferulic acid content. ACE inhibitory effect determined by HPLC was higher than 78%, being correlated with catechin and -coumaric acid. Therefore, propolis could be useful for food, pharmaceutical, and cosmetic companies, also helping to reduce risk factors for diseases related to oxidative damage, inflammatory processes, and hypertension. This research also highlights the necessity for harmonized analysis methods and the expression of results for propolis.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox9010075