Ligament/Tendon Culture under Hypoxic Conditions: A Systematic Review

The hypoxic environment is a substantial factor in maintenance, proliferation, and differentiation of the cell cultures. Low oxygen is known as a potent chondrogenesis stimulus in stem cells that is important for clinical application and engineering of functional cartilage. Hypoxia can potentially i...

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Bibliographic Details
Published in:Advanced pharmaceutical bulletin 2021-09, Vol.11 (4), p.595-600
Main Authors: Rhatomy, Sholahuddin, Utomo, Dwikora Novembri, Prakoeswa, Cita Rosita Sigit, Rantam, Fedik Abdul, Suroto, Heri, Mahyudin, Ferdiansyah
Format: Article
Language:English
Subjects:
Cell culture
Cell growth
culture
Cytokines
Hypoxia
hypoxic condition
Laboratory animals
ligament
Ligaments
Performance evaluation
Review
Stem cells
Systematic review
tendon
Tendons
Tumor necrosis factor-TNF
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Summary:The hypoxic environment is a substantial factor in maintenance, proliferation, and differentiation of the cell cultures. Low oxygen is known as a potent chondrogenesis stimulus in stem cells that is important for clinical application and engineering of functional cartilage. Hypoxia can potentially induce angiogenesis process by secretion of cytokines. This systematic review goal is to discover the effect of hypoxic condition on tendon/ ligament culture and the best oxygen level of hypoxia for in vitro and in vivo studies. We included 21 articles. A comprehensive review of this database confirms that the hypoxic condition is a substantial factor in the maintenance, proliferation, and differentiation of ligament/tendon cultures. Cell proliferation in the severe hypoxic (oxygen concentration of 1%) group at 24 h postcultivation was considered significant, but cell proliferation was markedly inhibited in the severe hypoxic group after 48 h.
ISSN:2228-5881
2251-7308
DOI:10.34172/apb.2021.069