Inhibition of SARS-CoV-2 Virus Entry by the Crude Polysaccharides of Seaweeds and Abalone Viscera In Vitro

• Published in: Marine drugs 2021-04, Vol.19 (4), p.219
• Main Authors: Yim, Sung-Kun, Kim, Kian, Kim, In-Hee, Chun, Sang-Ho, Oh, Tae-Hwan, Kim, Jin-Ung, Kim, Jung-Won, Jung, Woo-Huk, Moon, Ho-Sang, Ku, Bo-Sung, Jung, Kyoo-Jin
• Format: Article
• Language: English
• Subjects: abalone viscera; ACE2; Algae; Angiotensin-converting enzyme 2; Animals; Antiviral activity; Antiviral agents; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Carbohydrates; Coronaviruses; COVID-19; COVID-19 - virology; COVID-19 vaccines; Cytotoxicity; Dietary minerals; Fucoidan; Gastropoda - chemistry; Glycoproteins; HEK293 Cells; HIV; Human immunodeficiency virus; Humans; Influenza; Inhibitory Concentration 50; Marine molluscs; Molecular weight; polysaccharide; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Porphyra; Proteins; Saccharides; SARS-CoV-2; SARS-CoV-2 - physiology; seaweed; Seaweed - chemistry; Seaweeds; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Viral diseases; Viral infections; Virus Internalization - drug effects; Viruses; Viscera
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md19040219

Much attention is being devoted to the potential of marine sulfated polysaccharides as antiviral agents in preventing COVID-19. In this study, sulfated fucoidan and crude polysaccharides, extracted from six seaweed species (Undaria pinnatifida sporophyll, Laminaria japonica, Hizikia fusiforme, Sargassum horneri, Codium fragile, Porphyra tenera) and Haliotis discus hannai (abalone viscera), were screened for their inhibitory activity against SARS-CoV-2 virus entry. Most of them showed significant antiviral activities at an IC50 of 12~289 μg/mL against SARS-CoV-2 pseudovirus in HEK293/ACE2, except for P. tenera (IC50 > 1000 μg/mL). The crude polysaccharide of S. horneri showed the strongest antiviral activity, with an IC50 of 12 μg/mL, to prevent COVID-19 entry, and abalone viscera and H. fusiforme could also inhibit SARS-CoV-2 infection with an IC50 of 33 μg/mL and 47 μg/mL, respectively. The common properties of these crude polysaccharides, which have strong antiviral activity, are high molecular weight (>800 kDa), high total carbohydrate (62.7~99.1%), high fucose content (37.3~66.2%), and highly branched polysaccharides. These results indicated that the crude polysaccharides from seaweeds and abalone viscera can effectively inhibit SARS-CoV-2 entry.