The Role of BMP7 in the Proliferation of Hu Sheep Dermal Papilla Cells Is Influenced by DNA Methylation

Previous studies have shown that the gene is differentially expressed in Hu sheep lamb skin of different pattern types, and its expression level is significantly correlated with hair follicle indices of different pattern types, but the molecular mechanism of the differential expression of the gene r...

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Bibliographic Details
Published in:Animals (Basel) 2024-06, Vol.14 (11), p.1699
Main Authors: Lv, Xiaoyang, He, Mingliang, Wang, Shanhe, Zheng, Wenxin, Zhou, Hanlin, Mwacharo, Joram M, Sun, Wei
Format: Article
Language:English
Subjects:
Asymmetry
BMP7 gene
dermal papilla cell
DNA
DNA methylation
DNA polymerase
Follicles
Gene expression
Genes
Hu sheep
lamb skin
Methylation
Plasmids
Sheep
Skin
Wool
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Summary:Previous studies have shown that the gene is differentially expressed in Hu sheep lamb skin of different pattern types, and its expression level is significantly correlated with hair follicle indices of different pattern types, but the molecular mechanism of the differential expression of the gene remains unclear. This study investigated the effect of DNA methylation on the transcriptional expression of . Firstly, we found that the mRNA expression of the BMP7 gene and the activity of the core promoter of the BMP7 gene were upregulated after 5-Aza-Deoxycytidine-induced demethylation treatment using qRT-PCR and double luciferase reporter assay. Then, we found that the proliferation of Hu sheep DPCs in vitro was promoted after 5-Aza-Deoxycytidine-induced demethylation treatment through qRT-PCR, CCK-8, and EdU assay, and that the overexpression of DNMT1 in DPCs induced the opposite effect. In addition, the results of the cell cycle assay reveal that the percentage of cells in the S phase was increased after 5-Aza-Deoxycytidine-induced demethylation treatment, and that the percentage of cells in the S phase was decreased after overexpression of DNMT1 in DPCs. This study indicated that the differential expression of the gene in different patterns of Hu sheep lamb skin may be regulated by DNA methylation modification. In addition, DNA methylation can regulate the proliferation and cell cycle of DPCs in Hu sheep.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani14111699