Lactobacillus johnsonii CJLJ103 attenuates colitis and memory impairment in mice by inhibiting gut microbiota lipopolysaccharide production and NF-κB activation

• Published in: Journal of functional foods 2017-07, Vol.34, p.359-368
• Main Authors: Lim, Su-Min, Jang, Hyo-Min, Jeong, Jin-Ju, Han, Myung Joo, Kim, Dong-Hyun
• Format: Article
• Language: English
• Subjects: Colitis; Gut microbitoa; Lactobacillus johnsonii; Lipopolysaccharide; Memory impairment; NF-κB activation
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2017.05.016

•Lactobacillus johnsonii CJLJ103 (LJ) inhibited E. coli growth and its LPS production.•LJ increased the expression of tight junction proteins in Caco-2 cells and mice with TNBS-induced colitis (TIC).•LJ inhibited fecal LPS level and proteobacteria/bacteroidetes ratio in mice with TIC.•Anti-colitic effect of live LJ was not significantly different from that of heat-killed LJ.•LJ attenuated LPS-induced memory impairment and hippocampal NF-κB activation. Exposure to excessive lipopolysaccharide (LPS) by the gut microbiota disturbance causes ulcerative colitis and memory impairment. Therefore, we isolated anti-inflammatory Lactobacillus johnsonii CJLJ103 (LJ) from human fecal microbiota as an inhibitor for Escherichia coli growth and LPS production and investigated anti-colitic and memory impairment-ameliorating effects in mice. Oral administration of LJ inhibited 2,3,6-trinitrobenzenesulfonic acid (TNBS)-induced colon shortening, colonic myeloperoxidase activity, and NF-κB activation in mice. Furthermore, LJ inhibited TNBS-induced TNF and IL-1β expression in the colon but increased TNBS-suppressed expression of IL-10 and tight-junction proteins. Treatment with LJ inhibited fecal LPS levels and proteobacteria/bacteroidetes ratio in mice with TNBS-induced colitis. Furthermore, LJ ameliorated LPS-induced memory impairment in mice. LJ also suppressed LPS-induced NF-κB activation in the hippocampus and increased LPS-suppressed brain-derived neurotrophic factor expression. These findings suggest that LJ, a member of human gut microbiota, may ameliorate colitis and memory impairment, thereby acting as a useful probiotic.