Comparative analysis of freshwater phytoplankton communities in two lakes of Burabay National Park using morphological and molecular approaches

We analyzed phytoplankton assemblages’ variations in oligo-mesotrophic Shchuchie and Burabay lakes using traditional morphological and next-generation sequencing (NGS) approaches. The total phytoplankton biodiversity and abundance estimated by both microscopy and NGS were significantly higher in Lak...

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Bibliographic Details
Published in:Scientific reports 2021-08, Vol.11 (1), p.16130-16130, Article 16130
Main Authors: Malashenkov, Dmitry V., Dashkova, Veronika, Zhakupova, Kymbat, Vorobjev, Ivan A., Barteneva, Natasha S.
Format: Article
Language:English
Subjects:
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Algae
Aquatic ecosystems
Biodiversity
Chloroplasts
Comparative analysis
Ecosystem
Flow Cytometry
Freshwater lakes
Gene Library
Genera
High-Throughput Nucleotide Sequencing
Homology
Humanities and Social Sciences
Instrumentation
Kazakhstan
Lakes
Lakes - microbiology
Microscopy
Microscopy, Phase-Contrast
Morphology
multidisciplinary
National parks
Next-generation sequencing
Parks, Recreational
Phytoplankton
Phytoplankton - classification
Phytoplankton - genetics
Phytoplankton - ultrastructure
Plankton
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 18S - genetics
rRNA 16S
rRNA 18S
Science
Science (multidisciplinary)
Species composition
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Summary:We analyzed phytoplankton assemblages’ variations in oligo-mesotrophic Shchuchie and Burabay lakes using traditional morphological and next-generation sequencing (NGS) approaches. The total phytoplankton biodiversity and abundance estimated by both microscopy and NGS were significantly higher in Lake Burabay than in Lake Shchuchie. NGS of 16S and 18S rRNA amplicons adequately identify phytoplankton taxa only on the genera level, while species composition obtained by microscopic examination was significantly larger. The limitations of NGS analysis could be related to insufficient coverage of freshwater lakes phytoplankton by existing databases, short algal sequences available from current instrumentation, and high homology of chloroplast genes in eukaryotic cells. However, utilization of NGS, together with microscopy allowed us to perform a complete taxonomic characterization of phytoplankton lake communities including picocyanobacteria, often overlooked by traditional microscopy. We demonstrate the high potential of an integrated morphological and molecular approach in understanding the processes of organization in aquatic ecosystem assemblages.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-95223-z