Effects of the In Ovo and Dietary Supplementation of L-Ascorbic Acid on the Growth Performance, Inflammatory Response, and Eye L-Ascorbic Acid Concentrations in Ross 708 Broiler Chickens

• Published in: Animals (Basel) 2022-09, Vol.12 (19), p.2573
• Main Authors: Mousstaaid, Ayoub, Fatemi, Seyed Abolghasem, Elliott, Katie Elaine Collins, Levy, April Waguespack, Miller, William Wadd, Gerard, Patrick D, Alqhtani, Abdulmohsen Hussen, Peebles, Edgar David
• Format: Article
• Language: English
• Subjects: Amino acids; Antioxidants; Ascorbic acid; Birds; Bone growth; Chicks; Conversion ratio; Diet; Dietary intake; Efficiency; Eggs; Embryos; eye L-AA concentration; Feed conversion; growth performance; Hatching; in ovo injection; Incubation; Inflammation; Inflammatory response; Injection; Injections; Juveniles; L-ascorbic acid; Males; Mortality; Nitric oxide; Osteogenesis; Plasma; Poultry; Vitamin C
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani12192573

Effects of the dietary and in ovo administration of L-ascorbic acid (L-AA) on the performance, plasma nitric oxide, and eye L-AA concentrations of Ross 708 broilers were investigated. At 17 days of incubation, live embryonated hatching eggs were randomly assigned to a non-injected or sham-injected (100 μL of saline) control group, or a group injected with either 12 or 25 mg of L-AA suspended in 100 μL of saline. Chicks received a commercial diet with or without 200 mg/kg of supplemental L-AA and were randomly assigned to each of 6 replicate floor pens in each in ovo injection-dietary treatment combination. Weekly live performance variables through 14 days of post hatch age (doa) and the eye weights in both sexes at 0, 7, and 14 doa were determined. At 0 and 14 doa, plasma nitric oxide levels and eye L-AA concentrations of one bird of each sex in each pen were determined. Dietary supplemental L-AA decreased feed intake and growth between 0 and 7 doa, but from 8 to 14 doa; all birds fed supplemental L-AA had a lower feed conversion ratio. At 14 doa, male chicks had higher eye L-AA concentrations and lower plasma nitric oxide levels when treated in ovo with 12 mg of L-AA. In conclusion, dietary L-AA may be used to improve feed conversion in the second week of broiler post hatch growth. However, the in ovo administration of 12 mg of L-AA can increase male eye L-AA concentrations and is effective in reducing their general inflammatory response.