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Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness
Plants respond to environmental cues adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, ad...
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Published in: | Frontiers in plant science 2021-10, Vol.12, p.686274-686274 |
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creator | Bharadwaj, Revuru Noceda, Carlos Mohanapriya, Gunasekharan Kumar, Sarma Rajeev Thiers, Karine Leitão Lima Costa, José Hélio Macedo, Elisete Santos Kumari, Aprajita Gupta, Kapuganti Jagadis Srivastava, Shivani Adholeya, Alok Oliveira, Manuela Velada, Isabel Sircar, Debabrata Sathishkumar, Ramalingam Arnholdt-Schmitt, Birgit |
description | Plants respond to environmental cues
adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This
underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at the systems level. Plants require oxygen-dependent respiration for energy-dependent adaptive morphology, such as germination, root and shoot growth, and formation of adventitious, clonal, and reproductive organs, fruits, and seeds. Fermentative paths can help in acclimation and, to our view, the role of alternative oxidase (AOX) in coordinating complex metabolic and physiological adjustments is underestimated. Cellular levels of sucrose are an important sensor of environmental stress. We explored the role of exogenous sucrose and its interplay with AOX during early seed germination. We found that sucrose-dependent initiation of fermentation during the first 12 h after imbibition (HAI) was beneficial to germination. However, parallel upregulated
expression was essential to control negative effects by prolonged sucrose treatment. Early downregulated
activity until 12 HAI improved germination efficiency in the absence of sucrose but suppressed early germination in its presence. The results also suggest that seeds inoculated with arbuscular mycorrhizal fungi (AMF) can buffer sucrose stress during germination to restore normal respiration more efficiently. Following this approach, we propose a simple method to identify organic seeds and low-cost
perspectives for early identifying disease tolerance, predicting plant holobiont behavior, and improving germination. Furthermore, the research strengthens the view that AOX can serve as a powerful functional marker source for seed hologenomes. |
doi_str_mv | 10.3389/fpls.2021.686274 |
format | article |
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adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This
underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at the systems level. Plants require oxygen-dependent respiration for energy-dependent adaptive morphology, such as germination, root and shoot growth, and formation of adventitious, clonal, and reproductive organs, fruits, and seeds. Fermentative paths can help in acclimation and, to our view, the role of alternative oxidase (AOX) in coordinating complex metabolic and physiological adjustments is underestimated. Cellular levels of sucrose are an important sensor of environmental stress. We explored the role of exogenous sucrose and its interplay with AOX during early seed germination. We found that sucrose-dependent initiation of fermentation during the first 12 h after imbibition (HAI) was beneficial to germination. However, parallel upregulated
expression was essential to control negative effects by prolonged sucrose treatment. Early downregulated
activity until 12 HAI improved germination efficiency in the absence of sucrose but suppressed early germination in its presence. The results also suggest that seeds inoculated with arbuscular mycorrhizal fungi (AMF) can buffer sucrose stress during germination to restore normal respiration more efficiently. Following this approach, we propose a simple method to identify organic seeds and low-cost
perspectives for early identifying disease tolerance, predicting plant holobiont behavior, and improving germination. Furthermore, the research strengthens the view that AOX can serve as a powerful functional marker source for seed hologenomes.</description><identifier>ISSN: 1664-462X</identifier><identifier>EISSN: 1664-462X</identifier><identifier>DOI: 10.3389/fpls.2021.686274</identifier><identifier>PMID: 34659277</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>bacterial endophytes and mycorrhizal fungi ; biotic stress ; organic seeds ; Plant Science ; ROS ; seed quality ; Warburg effect</subject><ispartof>Frontiers in plant science, 2021-10, Vol.12, p.686274-686274</ispartof><rights>Copyright © 2021 Bharadwaj, Noceda, Mohanapriya, Kumar, Thiers, Costa, Macedo, Kumari, Gupta, Srivastava, Adholeya, Oliveira, Velada, Sircar, Sathishkumar and Arnholdt-Schmitt.</rights><rights>Copyright © 2021 Bharadwaj, Noceda, Mohanapriya, Kumar, Thiers, Costa, Macedo, Kumari, Gupta, Srivastava, Adholeya, Oliveira, Velada, Sircar, Sathishkumar and Arnholdt-Schmitt. 2021 Bharadwaj, Noceda, Mohanapriya, Kumar, Thiers, Costa, Macedo, Kumari, Gupta, Srivastava, Adholeya, Oliveira, Velada, Sircar, Sathishkumar and Arnholdt-Schmitt</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-d91c074924c4e0a6ef3a7f83efb82d6c4f8b323df58b2e58d0a41f0a52cc70953</citedby><cites>FETCH-LOGICAL-c462t-d91c074924c4e0a6ef3a7f83efb82d6c4f8b323df58b2e58d0a41f0a52cc70953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518632/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518632/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34659277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bharadwaj, Revuru</creatorcontrib><creatorcontrib>Noceda, Carlos</creatorcontrib><creatorcontrib>Mohanapriya, Gunasekharan</creatorcontrib><creatorcontrib>Kumar, Sarma Rajeev</creatorcontrib><creatorcontrib>Thiers, Karine Leitão Lima</creatorcontrib><creatorcontrib>Costa, José Hélio</creatorcontrib><creatorcontrib>Macedo, Elisete Santos</creatorcontrib><creatorcontrib>Kumari, Aprajita</creatorcontrib><creatorcontrib>Gupta, Kapuganti Jagadis</creatorcontrib><creatorcontrib>Srivastava, Shivani</creatorcontrib><creatorcontrib>Adholeya, Alok</creatorcontrib><creatorcontrib>Oliveira, Manuela</creatorcontrib><creatorcontrib>Velada, Isabel</creatorcontrib><creatorcontrib>Sircar, Debabrata</creatorcontrib><creatorcontrib>Sathishkumar, Ramalingam</creatorcontrib><creatorcontrib>Arnholdt-Schmitt, Birgit</creatorcontrib><title>Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness</title><title>Frontiers in plant science</title><addtitle>Front Plant Sci</addtitle><description>Plants respond to environmental cues
adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This
underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at the systems level. Plants require oxygen-dependent respiration for energy-dependent adaptive morphology, such as germination, root and shoot growth, and formation of adventitious, clonal, and reproductive organs, fruits, and seeds. Fermentative paths can help in acclimation and, to our view, the role of alternative oxidase (AOX) in coordinating complex metabolic and physiological adjustments is underestimated. Cellular levels of sucrose are an important sensor of environmental stress. We explored the role of exogenous sucrose and its interplay with AOX during early seed germination. We found that sucrose-dependent initiation of fermentation during the first 12 h after imbibition (HAI) was beneficial to germination. However, parallel upregulated
expression was essential to control negative effects by prolonged sucrose treatment. Early downregulated
activity until 12 HAI improved germination efficiency in the absence of sucrose but suppressed early germination in its presence. The results also suggest that seeds inoculated with arbuscular mycorrhizal fungi (AMF) can buffer sucrose stress during germination to restore normal respiration more efficiently. Following this approach, we propose a simple method to identify organic seeds and low-cost
perspectives for early identifying disease tolerance, predicting plant holobiont behavior, and improving germination. Furthermore, the research strengthens the view that AOX can serve as a powerful functional marker source for seed hologenomes.</description><subject>bacterial endophytes and mycorrhizal fungi</subject><subject>biotic stress</subject><subject>organic seeds</subject><subject>Plant Science</subject><subject>ROS</subject><subject>seed quality</subject><subject>Warburg effect</subject><issn>1664-462X</issn><issn>1664-462X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkk1r3DAQhk1pacI2956Kjr3sVl-W7Uth2W7SQEog3UJvYlYe7SrYliPZoflz_W2V4zQkuozQvPPMaHiz7COjKyHK6ovtm7jilLOVKhUv5JvslCkll1Lx329f3E-ysxhvaTo5pVVVvM9OhFR5xYviNPu7rqEf3D2SG-yDPwRoW9cdyLcxTGELoXkgPxFrcoEhZWBwvkvau9EFjGSHbe8DBJdU2-4InUnK86TEbniULtdkE9zgDDTkxjdIrA9k3QwYJlRqe_3H1RCn9oexmem7Iwxk4xMrdDGJoyc_nAl-7_wAZGstmqm0wxg_ZO8sNBHPnuIi-3W-3W2-L6-uLy4366ulSRsYlnXFDC1kxaWRSEGhFVDYUqDdl7xWRtpyL7iobV7uOeZlTUEySyHnxhS0ysUiu5y5tYdb3QfXQnjQHpx-fPDhoCGkXzaoE8AWrGAgpZUoEGgtCpkDS6thilWJ9XVm9eO-xdqkVQVoXkFfZzp31Ad_r8uclSqNucg-PwGCvxsxDrp10WDTQId-jJrnpRCMK0aTlM7StL4YA9rnNozqyUV6cpGeXKRnF6WSTy_Hey747xnxD164yfE</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Bharadwaj, Revuru</creator><creator>Noceda, Carlos</creator><creator>Mohanapriya, Gunasekharan</creator><creator>Kumar, Sarma Rajeev</creator><creator>Thiers, Karine Leitão Lima</creator><creator>Costa, José Hélio</creator><creator>Macedo, Elisete Santos</creator><creator>Kumari, Aprajita</creator><creator>Gupta, Kapuganti Jagadis</creator><creator>Srivastava, Shivani</creator><creator>Adholeya, Alok</creator><creator>Oliveira, Manuela</creator><creator>Velada, Isabel</creator><creator>Sircar, Debabrata</creator><creator>Sathishkumar, Ramalingam</creator><creator>Arnholdt-Schmitt, Birgit</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20211001</creationdate><title>Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness</title><author>Bharadwaj, Revuru ; Noceda, Carlos ; Mohanapriya, Gunasekharan ; Kumar, Sarma Rajeev ; Thiers, Karine Leitão Lima ; Costa, José Hélio ; Macedo, Elisete Santos ; Kumari, Aprajita ; Gupta, Kapuganti Jagadis ; Srivastava, Shivani ; Adholeya, Alok ; Oliveira, Manuela ; Velada, Isabel ; Sircar, Debabrata ; Sathishkumar, Ramalingam ; Arnholdt-Schmitt, Birgit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-d91c074924c4e0a6ef3a7f83efb82d6c4f8b323df58b2e58d0a41f0a52cc70953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>bacterial endophytes and mycorrhizal fungi</topic><topic>biotic stress</topic><topic>organic seeds</topic><topic>Plant Science</topic><topic>ROS</topic><topic>seed quality</topic><topic>Warburg effect</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bharadwaj, Revuru</creatorcontrib><creatorcontrib>Noceda, Carlos</creatorcontrib><creatorcontrib>Mohanapriya, Gunasekharan</creatorcontrib><creatorcontrib>Kumar, Sarma Rajeev</creatorcontrib><creatorcontrib>Thiers, Karine Leitão Lima</creatorcontrib><creatorcontrib>Costa, José Hélio</creatorcontrib><creatorcontrib>Macedo, Elisete Santos</creatorcontrib><creatorcontrib>Kumari, Aprajita</creatorcontrib><creatorcontrib>Gupta, Kapuganti Jagadis</creatorcontrib><creatorcontrib>Srivastava, Shivani</creatorcontrib><creatorcontrib>Adholeya, Alok</creatorcontrib><creatorcontrib>Oliveira, Manuela</creatorcontrib><creatorcontrib>Velada, Isabel</creatorcontrib><creatorcontrib>Sircar, Debabrata</creatorcontrib><creatorcontrib>Sathishkumar, Ramalingam</creatorcontrib><creatorcontrib>Arnholdt-Schmitt, Birgit</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Frontiers in plant science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bharadwaj, Revuru</au><au>Noceda, Carlos</au><au>Mohanapriya, Gunasekharan</au><au>Kumar, Sarma Rajeev</au><au>Thiers, Karine Leitão Lima</au><au>Costa, José Hélio</au><au>Macedo, Elisete Santos</au><au>Kumari, Aprajita</au><au>Gupta, Kapuganti Jagadis</au><au>Srivastava, Shivani</au><au>Adholeya, Alok</au><au>Oliveira, Manuela</au><au>Velada, Isabel</au><au>Sircar, Debabrata</au><au>Sathishkumar, Ramalingam</au><au>Arnholdt-Schmitt, Birgit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness</atitle><jtitle>Frontiers in plant science</jtitle><addtitle>Front Plant Sci</addtitle><date>2021-10-01</date><risdate>2021</risdate><volume>12</volume><spage>686274</spage><epage>686274</epage><pages>686274-686274</pages><issn>1664-462X</issn><eissn>1664-462X</eissn><abstract>Plants respond to environmental cues
adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This
underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at the systems level. Plants require oxygen-dependent respiration for energy-dependent adaptive morphology, such as germination, root and shoot growth, and formation of adventitious, clonal, and reproductive organs, fruits, and seeds. Fermentative paths can help in acclimation and, to our view, the role of alternative oxidase (AOX) in coordinating complex metabolic and physiological adjustments is underestimated. Cellular levels of sucrose are an important sensor of environmental stress. We explored the role of exogenous sucrose and its interplay with AOX during early seed germination. We found that sucrose-dependent initiation of fermentation during the first 12 h after imbibition (HAI) was beneficial to germination. However, parallel upregulated
expression was essential to control negative effects by prolonged sucrose treatment. Early downregulated
activity until 12 HAI improved germination efficiency in the absence of sucrose but suppressed early germination in its presence. The results also suggest that seeds inoculated with arbuscular mycorrhizal fungi (AMF) can buffer sucrose stress during germination to restore normal respiration more efficiently. Following this approach, we propose a simple method to identify organic seeds and low-cost
perspectives for early identifying disease tolerance, predicting plant holobiont behavior, and improving germination. Furthermore, the research strengthens the view that AOX can serve as a powerful functional marker source for seed hologenomes.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>34659277</pmid><doi>10.3389/fpls.2021.686274</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | bacterial endophytes and mycorrhizal fungi biotic stress organic seeds Plant Science ROS seed quality Warburg effect |
title | Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness |
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