Phylogenetic Studies of Coxiella -Like Bacteria and Spotted Fever Group Rickettsiae in Ticks Collected From Vegetation in Chaiyaphum Province, Thailand

Ticks can transmit a wide variety of pathogens, including bacteria. Here, we report the detection of tick-associated bacteria in Chaiyaphum Province, northeastern Thailand. There have been few reports of tick-borne bacterial pathogens in the study areas, which are evergreen forests dominated by plat...

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Bibliographic Details
Published in:Frontiers in veterinary science 2022-04, Vol.9, p.849893-849893
Main Authors: Usananan, Pawiga, Kaenkan, Warissara, Sudsangiem, Ronnayuth, Baimai, Visut, Trinachartvanit, Wachareeporn, Ahantarig, Arunee
Format: Article
Language:English
Subjects:
Amblyomma
Coxiella-like bacteria
Haemaphysalis
Rickettsia
tick
Veterinary Science
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Summary:Ticks can transmit a wide variety of pathogens, including bacteria. Here, we report the detection of tick-associated bacteria in Chaiyaphum Province, northeastern Thailand. There have been few reports of tick-borne bacterial pathogens in the study areas, which are evergreen forests dominated by plateaus at elevations of approximately 1,000 m. In total, 94 ticks were collected from vegetation. They were screened for the presence of , and bacteria using PCR assays. In this study, we found ticks from two genera, and , that were positive for -like bacteria (CLB) and . and spp. were not detected in these two tick genera. The results revealed the evolutionary relationships of CLB in , and ticks using the rRNA and markers, which clustered together with known isolates of ticks from the same genera. In contrast, the marker showed different results. On the basis of the phylogenetic analysis and BLAST results, three groups of CLB were found: (1) CLB from grouped as a sister clade to CLB from ; (2) CLB from was distantly related to CLB from ; and (3) CLB from grouped as sister clade to CLB from from French Guiana and Brazil. For studies, phylogenetic trees of the , and genes revealed two groups of Spotted Fever Group (SFG) : (1) SFG that formed a sister clade with AT-1 (belong to the subgroup) in and (2) SFG that formed a distantly related group to 3-7-female6-CWPP (belong to the subgroup) in . This study expanded our knowledge of the diversity of tick-borne and bacteria. The pathogenic roles of these bacteria also need to be investigated further.
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2022.849893