Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland

Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2014-07, Vol.109 (4), p.436-441
Main Authors: Paluchowska, Paulina, Tokarczyk, Marianna, Bogusz, Bozena, Skiba, Iwona, Budak, Alicja
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antifungal Agents - pharmacology
Candida albicans
Candida albicans - drug effects
Candida albicans - genetics
Candida glabrata
Candida glabrata - drug effects
Candida glabrata - genetics
Candidiasis - epidemiology
Candidiasis - microbiology
DNA Primers - genetics
DNA, Fungal - genetics
epidemiology
Female
genotyping
Humans
Intensive Care Units
Male
Microbial Sensitivity Tests
Middle Aged
Molecular Typing
PARASITOLOGY
Poland - epidemiology
Random Amplified Polymorphic DNA Technique
RAPD PCR
TROPICAL MEDICINE
Young Adult
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Summary:Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.
ISSN:1678-8060
0074-0276
1678-8060
0074-0276
DOI:10.1590/0074-0276140099