Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model

Objective. In the present study, since PON1 is known as an HDL-associated antioxidant enzyme that inhibits the oxidative modification of LDL and oxidative stress plays a role in the pathogenesis of mesenteric ischemia, we investigated the changes in PON1 activity and lipid profile in an experimental...

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Published in:Gastroenterology research and practice 2012-01, Vol.2012 (2012), p.1-5
Main Authors: Unal, Ethem, Eris, Cengiz, Kaya, Bülent, Uzun, Hafize, Cavdar, Faruk, Yildar, Murat, Kiziler, Ali Riza, Aydemir, Birsen, Gunes, Pembegul, Kutanis, Riza, Titiz, Izzet
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Atherosclerosis
Biophysics
Cardiovascular disease
Cholesterol
Colon
Diabetes
Enzymes
Free radicals
Hospitals
Inflammatory bowel disease
Ischemia
Laboratories
Lipid peroxidation
Lipids
Metabolic disorders
Mortality
Oxidative stress
Pathogenesis
Rodents
Studies
Surgery
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Summary:Objective. In the present study, since PON1 is known as an HDL-associated antioxidant enzyme that inhibits the oxidative modification of LDL and oxidative stress plays a role in the pathogenesis of mesenteric ischemia, we investigated the changes in PON1 activity and lipid profile in an experimental ischemic colitis model. Methods. Forty male Wistar albino rats were divided into two groups: the control group (N=15) and the experimental group (N=25). All animals were anesthetized with ether and ketamine anesthesia to undergo a midline laparotomy. Ischemic colitis was induced by marginal vessel ligation in the splenic flexura (devascularization process). A sham laparotomy was performed in the control group. All animals were sacrificed on the seventh postoperative day. Oxidative stress marker (malonyldialdehyde, MDA), lipid profile, and paraoxonase (PON-1) and arylesterase activities were determined. Histopathological evaluation was done under light microscopy, after sectioning and staining with hematoxyline and eosin. Statistical analysis was conducted using Student’s t-test and Mann-Whitney U test, and P
ISSN:1687-6121
1687-630X
DOI:10.1155/2012/979506