sandwich enzyme-linked immunosorbent assay for human plasma apolipoprotein A-V concentration

Apolipoprotein A-V (apoA-V) is a recently discovered apolipoprotein that appears to have a role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20 ng/ml. The ELISA was then u...

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Bibliographic Details
Published in:Journal of lipid research 2005-09, Vol.46 (9), p.2015-2022
Main Authors: Ishihara, Mitsuaki, Kujiraoka, Takeshi, Iwasaki, Tadao, Nagano, Makoto, Takano, Mayumi, Ishii, Jun, Tsuji, Masahiro, Ide, Hajime, Miller, Irina P, Miller, Norman E, Hattori, Hiroaki
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Apolipoprotein A-I - blood
Apolipoprotein A-V
Apolipoproteins - blood
Apolipoproteins - genetics
Apolipoproteins A
Apolipoproteins E - blood
Cholesterol, HDL - blood
diabetes mellitus
Diabetes Mellitus, Type 2 - blood
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
immunoassay
Male
Polymorphism, Single Nucleotide
Reference Values
Sex Characteristics
single nucleotide polymorphism
triglyceride
Triglycerides - blood
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Summary:Apolipoprotein A-V (apoA-V) is a recently discovered apolipoprotein that appears to have a role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20 ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106 patients with insulin-resistant diabetes mellitus. In the healthy subjects, total apoA-V concentration was 179.2 ± 74.8 ng/ml, and it was greater in females than in males (P < 0.005). It was correlated positively with the plasma HDL cholesterol (r = 0.32, P < 0.0001), apoA-I (r = 0.27, P = 0.0001), and apoE (r = 0.18, P = 0.011) concentrations and negatively with plasma TG concentration (r = -0.22, P = 0.021). In relation to single nucleotide polymorphism 3 (-1131C/T) of the apoA-V gene, apoA-V concentration was higher in the T/T type than in the C/C type (P < 0.01). Plasma TG concentration was lower in the T/T type than in the C/C or C/T type (P < 0.05). ApoA-V concentration was lower in the diabetic patients (69.4 ± 44.3 ng/ml; P < 0.01) than in the healthy controls.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.D500018-JLR200