Mechanism of Crosstalk between the LSD1 Demethylase and HDAC1 Deacetylase in the CoREST Complex

• Published in: Cell reports (Cambridge) 2020-02, Vol.30 (8), p.2699-2711.e8
• Main Authors: Song, Yun, Dagil, Lisbeth, Fairall, Louise, Robertson, Naomi, Wu, Mingxuan, Ragan, T.J., Savva, Christos G., Saleh, Almutasem, Morone, Nobuhiro, Kunze, Micha B.A., Jamieson, Andrew G., Cole, Philip A., Hansen, D. Flemming, Schwabe, John W.R.
• Format: Article
• Language: English
• Subjects: Acetylation; Amino Acid Sequence; Animals; Co-Repressor Proteins - metabolism; CoREST complex; Cryoelectron Microscopy; Demethylation; HDAC1; HEK293 Cells; histone deacetylase; Histone Deacetylase 1 - metabolism; Histone Demethylases - metabolism; Humans; KDM1A; Kinetics; LSD1; lysine demethylase; Models, Molecular; Nerve Tissue Proteins - metabolism; nucleosome; Nucleosomes - metabolism; RCOR1; Xenopus
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2020.01.091

The transcriptional corepressor complex CoREST is one of seven histone deacetylase complexes that regulate the genome through controlling chromatin acetylation. The CoREST complex is unique in containing both histone demethylase and deacetylase enzymes, LSD1 and HDAC1, held together by the RCOR1 scaffold protein. To date, it has been assumed that the enzymes function independently within the complex. Now, we report the assembly of the ternary complex. Using both structural and functional studies, we show that the activity of the two enzymes is closely coupled and that the complex can exist in at least two distinct states with different kinetics. Electron microscopy of the complex reveals a bi-lobed structure with LSD1 and HDAC1 enzymes at opposite ends of the complex. The structure of CoREST in complex with a nucleosome reveals a mode of chromatin engagement that contrasts with previous models. [Display omitted] •The activities of LSD1 and HDAC1 are closely coupled in the CoREST complex•Both LSD1 and HDAC1 exist in two different kinetic states•CoREST has a bi-lobed, flexible structure with the two enzymes located at opposite ends•CoREST interacts with methylated nucleosomes via LSD1, but not HDAC1 or RCOR1 Using a real-time NMR assay, Song et al. characterize crosstalk between LSD1 and HDAC1 in the CoREST complex. Activation or inhibition of one enzyme strongly affects activity of the other. Electron microscopy studies of the complex reveal a bi-lobed structure with implications for the mode of interaction with nucleosomes.