Treatment of Murine Toxoplasmosis with Oral and Parenteral Artemether and Following by Detection of B1 Gene by Quantitative Real-Time PCR (qPCR) for Evaluating Parasite Density

Background: Toxoplasma gondiiis an obligate intracellular parasite that can infect humans and animals. As the choice drug have shown side effects, development a new drug with low toxicity will be necessary.Methods: BALB/c mice were infected with tachyzoiets of T. gondii. After treatment by oral and...

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Published in:Iranian journal of parasitology 2022-01, Vol.17 (1), p.53-61
Main Authors: Mikaeiloo, Hajar, Ghaffarifar, Fatemeh, Dalimi, Abdolhossein, Mohammad Zuhair Hassan, Sharifi, Zohreh
Format: Article
Language:English
Subjects:
Annealing
Artemether
BALB/c mice
Brain research
Cysts
Genes
Infections
Liver
Medical research
Oral
Original
Parasites
Parasitology
Parenteral
Polymerase chain reac-tion
Protozoa
Thermal cycling
Toxoplasma gondii
Toxoplasmosis
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Summary:Background: Toxoplasma gondiiis an obligate intracellular parasite that can infect humans and animals. As the choice drug have shown side effects, development a new drug with low toxicity will be necessary.Methods: BALB/c mice were infected with tachyzoiets of T. gondii. After treatment by oral and parenteral artemether (250 µg/mice) and sulfadiazine (50 µg/mice), we evaluated the rates of survival in treated and control mice. The fold change of B1 gene (target gene) expression in liver and brain of mice treated with parenteral artemether (i.p.), oral artemether (via gavage) and sulfadiazine, were detected by using the Real-Time quantitative PCR.Results: Both treatment with sulfadiazine and artemether showed significant prolongation in time to death of the infected mice compared to the control group. Median survival days for parenteral artemether, oral artemether, sulfadiazine and control group were 8, 11, 12 and 6 d respectively. Expression of B1 gene in liver and brain of mice after treatment with artemether and sulfadiazine were reduced in comparison to housekeeping gene (β-tubulin gene). The fold change (comparing to control group) for parenteral artemether, oral artemether, sulfadiazine is 0.034, 0.027 and 0.111 for liver and 0.220, 0.425 and 0.366 for brain respectively.Conclusion: Artemether is effective to control the tachyzoites of T. godii in vivo conditions and oral treatment is more effective than parenteral treatment. Due to its low cytotoxicity and its high effective action against the tachyzoietes of T. godii in susceptible animals.
ISSN:1735-7020
2008-238X
DOI:10.18502/ijpa.v17i1.9016