Loading…

In Vitro Potential of the Acetone Leaf Extract and Fractions of Psychotria capensis (Eckl.) Vatke (Rubiaceae) to Combat Co-Infection of Tuberculosis and Helminthiasis

Tuberculosis (TB) is a disease of global importance that affects millions of people. Approximately a quarter of the world's population is currently infected with , and about 10% of those infected will develop into active disease, particularly immune compromised individuals. Helminthiasis is of...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in pharmacology 2022-01, Vol.12, p.744137-744137
Main Authors: Aro, Abimbola O, Famuyide, Ibukun M, Oyagbemi, Ademola A, Kabongo-Kayoka, Prudence N, McGaw, Lyndy J
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tuberculosis (TB) is a disease of global importance that affects millions of people. Approximately a quarter of the world's population is currently infected with , and about 10% of those infected will develop into active disease, particularly immune compromised individuals. Helminthiasis is of global health importance, affecting over 2 billion people mostly in resource-poor countries. Co-infection with tuberculosis (TB) and helminths (worms) is an emerging global public health concern with both affecting about one-third of the global population. Chronic infection with helminths can result in impaired immune responses to TB as well as enhancing failure to TB therapy and BCG vaccination. Antimycobacterial and anthelmintic activities of the acetone extract and fractions of were evaluated, including their safety. In addition, the anti-inflammatory and immunomodulatory effect of the fractions and crude extract of were assessed. Antimycobacterial activity of the extract and fractions was tested against four non-tuberculous mycobacteria ( , , BCG) and pathogenic H37Rv while the Egg Hatch Assay (EHA) was used for the anthelmintic test on eggs of . Cytotoxicity was determined against Vero kidney cells while immune modulation via cytokine production was determined on activated macrophages. The minimum inhibitory concentration (MIC) values of the acetone extract and fractions ranged from 39 to 1,250 μg/ml with the crude extract and hexane fraction having the best MIC values (both 39 μg/ml). In the EHA, the inhibitory concentration (IC ) ranged from 160 to 630 μg/ml with the hexane fraction having the best activity. The hexane and chloroform fractions were relatively non-toxic with LC values of 290 and 248 μg/ml respectively, while the acetone crude extract (64 μg/ml) and n-butanol fraction (71 μg/ml) were moderately toxic. The SI values (LC /MIC) ranged from 0.1 to 7.4 with the hexane fraction having the highest value against (7.4). The hexane fraction had the best dual anthelmintic and antimycobacterial activity. This fraction had the best NO inhibitory activity and was the least cytotoxic, indicating that its activity was not due to general metabolic toxicity, with 96.54% cell viability. Pro-inflammatory cytokines such as IL-12p70 were upregulated while IL-10 expression was inhibited by the extracts. Compounds were detected using GC-MS analysis, and in both the crude acetone extract and the hexane fraction was the diterpene neophytadiene, which has anti-inflammator
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2021.744137