Analysis of the Phospholipid Profile of the Collection Strain PAO1 and Clinical Isolates of Pseudomonas aeruginosa in Relation to Their Attachment Capacity

Bacteria form multicellular and resistant structures named biofilms. Biofilm formation starts with the attachment phase, and the molecular actors involved in this phase, except adhesins, are poorly characterized. There is growing evidence that phospholipids are more than simple structural bricks. Th...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2021-04, Vol.22 (8), p.4003
Main Authors: Le Sénéchal, Caroline, Puges, Mathilde, Barthe, Christophe, Costaglioli, Patricia, Tokarski, Caroline, Buré, Corinne, Vilain, Sébastien
Format: Article
Language:English
Subjects:
Adhesins
Antibiotics
Bacteria
Bacterial Adhesion
Bacteriology
biofilm
Biofilms
Clinical isolates
clinical strain
Cystic fibrosis
Humans
Identification
Life Sciences
Lipidomics
Lungs
Mass spectrometry
Mass spectroscopy
Microbiology and Parasitology
Nosocomial infections
Pathogens
Phenotypes
phospholipid
Phospholipids
Phospholipids - metabolism
Principal Component Analysis
Pseudomonas aeruginosa
Pseudomonas aeruginosa - isolation & purification
Pseudomonas aeruginosa - physiology
Reproducibility of Results
Scientific imaging
Strains (organisms)
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacteria form multicellular and resistant structures named biofilms. Biofilm formation starts with the attachment phase, and the molecular actors involved in this phase, except adhesins, are poorly characterized. There is growing evidence that phospholipids are more than simple structural bricks. They are involved in bacterial adaptive physiology, but little is known about their role in biofilm formation. Here, we report a mass spectrometry analysis of the phospholipid (PL) profile of several strains of isolated from cystic fibrosis patients. The aim of our study was to evaluate a possible link between the PL profile of a strain and its attachment phenotype. Our results showed that PL profile is strongly strain-dependent. The PL profile of PAO1, a collection strain, was different from those of 10 clinical isolates characterized either by a very low or a very high attachment capacity. We observed also that the clinical strain's PL profiles varied even more importantly between isolates. By comparing groups of strains having similar attachment capacities, we identified one PL, PE 18:1-18:1, as a potential molecular actor involved in attachment, the first step in biofilm formation. This PL represents a possible target in the fight against biofilms.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22084003