Optogenetic dissection of transcriptional repression in a multicellular organism

Transcriptional control is fundamental to cellular function. However, despite knowing that transcription factors can repress or activate specific genes, how these functions are implemented at the molecular level has remained elusive, particularly in the endogenous context of developing animals. Here...

Full description

Saved in:
Bibliographic Details
Published in:Nature communications 2024-10, Vol.15 (1), p.9263-11, Article 9263
Main Authors: Zhao, Jiaxi, Lammers, Nicholas C., Alamos, Simon, Kim, Yang Joon, Martini, Gabriella, Garcia, Hernan G.
Format: Article
Language:English
Subjects:
631/136
631/1647/2253
631/553
631/57
Animals
Biology
Dissection
Embryos
Gene expression
Gene Expression Regulation
Gene loci
Gene regulation
Gene silencing
Genetics
Humanities and Social Sciences
In vivo methods and tests
Information processing
Mathematical models
multidisciplinary
Optics
Optogenetics
Proteins
Repressor Proteins - genetics
Repressor Proteins - metabolism
Science
Science (multidisciplinary)
Single-Cell Analysis
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
Zinc finger proteins
Zinc Fingers
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c378t-f8e2ddee73822fe01eebd81f912677ea28ef5cb424567814e96c3c2df251ea933
container_end_page 11
container_issue 1
container_start_page 9263
container_title Nature communications
container_volume 15
creator Zhao, Jiaxi
Lammers, Nicholas C.
Alamos, Simon
Kim, Yang Joon
Martini, Gabriella
Garcia, Hernan G.
description Transcriptional control is fundamental to cellular function. However, despite knowing that transcription factors can repress or activate specific genes, how these functions are implemented at the molecular level has remained elusive, particularly in the endogenous context of developing animals. Here, we combine optogenetics, single-cell live-imaging, and mathematical modeling to study how a zinc-finger repressor, Knirps, induces switch-like transitions into long-lived quiescent states. Using optogenetics, we demonstrate that repression is rapidly reversible (~1 min) and memoryless. Furthermore, we show that the repressor acts by decreasing the frequency of transcriptional bursts in a manner consistent with an equilibrium binding model. Our results provide a quantitative framework for dissecting the in vivo biochemistry of eukaryotic transcriptional regulation. Using optogenetics in the fly embryo, this study investigates how a transcriptional repressor drives switch-like, rapidly reversible repression by modulating transcriptional burst frequency, offering insights into gene regulation dynamics in development.
doi_str_mv 10.1038/s41467-024-53539-0
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_b3d19fd36d704ae2969e0a285f177189</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_b3d19fd36d704ae2969e0a285f177189</doaj_id><sourcerecordid>3121049847</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-f8e2ddee73822fe01eebd81f912677ea28ef5cb424567814e96c3c2df251ea933</originalsourceid><addsrcrecordid>eNp9kk1v1DAQhiMEolXbP9ADisSFS8BjO7F9QqgCWqlSe6Bny2uPF6-SONgJUv893mZbWg74YmvmmXc-PFV1DuQjECY_ZQ68Ew2hvGlZy1RDXlXHlHBoQFD2-tn7qDrLeUfKYQok52-rI6Z4B0rI4-r2ZprjFkecg61dyBntHOJYR1_PyYzZpjDtDaavE04Jc957w1ibelj6EoR9v_Qm1TFtzRjycFq98abPeHa4T6q7b19_XFw21zffry6-XDeWCTk3XiJ1DlEwSalHAogbJ8EroJ0QaKhE39oNp7zthASOqrPMUudpC2gUYyfV1arrotnpKYXBpHsdTdAPhlKONqnU16PeMAfKO9Y5QbhBqjqFpGRoPQgBUhWtz6vWtGwGdBbH0nv_QvSlZww_9Tb-1gAtMKBtUfhwUEjx14J51kPI-9mYEeOSdYGAdFwRXtD3_6C7uKQy4APFleSiUHSlbIo5J_RP1QDR-wXQ6wLosgD6YQE0KUHvnvfxFPL43QVgK5CLa9xi-pv7P7J_ADiwvIo</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3121049847</pqid></control><display><type>article</type><title>Optogenetic dissection of transcriptional repression in a multicellular organism</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central (Open access)</source><source>Nature</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Zhao, Jiaxi ; Lammers, Nicholas C. ; Alamos, Simon ; Kim, Yang Joon ; Martini, Gabriella ; Garcia, Hernan G.</creator><creatorcontrib>Zhao, Jiaxi ; Lammers, Nicholas C. ; Alamos, Simon ; Kim, Yang Joon ; Martini, Gabriella ; Garcia, Hernan G.</creatorcontrib><description>Transcriptional control is fundamental to cellular function. However, despite knowing that transcription factors can repress or activate specific genes, how these functions are implemented at the molecular level has remained elusive, particularly in the endogenous context of developing animals. Here, we combine optogenetics, single-cell live-imaging, and mathematical modeling to study how a zinc-finger repressor, Knirps, induces switch-like transitions into long-lived quiescent states. Using optogenetics, we demonstrate that repression is rapidly reversible (~1 min) and memoryless. Furthermore, we show that the repressor acts by decreasing the frequency of transcriptional bursts in a manner consistent with an equilibrium binding model. Our results provide a quantitative framework for dissecting the in vivo biochemistry of eukaryotic transcriptional regulation. Using optogenetics in the fly embryo, this study investigates how a transcriptional repressor drives switch-like, rapidly reversible repression by modulating transcriptional burst frequency, offering insights into gene regulation dynamics in development.</description><identifier>ISSN: 2041-1723</identifier><identifier>EISSN: 2041-1723</identifier><identifier>DOI: 10.1038/s41467-024-53539-0</identifier><identifier>PMID: 39461978</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/136 ; 631/1647/2253 ; 631/553 ; 631/57 ; Animals ; Biology ; Dissection ; Embryos ; Gene expression ; Gene Expression Regulation ; Gene loci ; Gene regulation ; Gene silencing ; Genetics ; Humanities and Social Sciences ; In vivo methods and tests ; Information processing ; Mathematical models ; multidisciplinary ; Optics ; Optogenetics ; Proteins ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Science ; Science (multidisciplinary) ; Single-Cell Analysis ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Zinc finger proteins ; Zinc Fingers</subject><ispartof>Nature communications, 2024-10, Vol.15 (1), p.9263-11, Article 9263</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c378t-f8e2ddee73822fe01eebd81f912677ea28ef5cb424567814e96c3c2df251ea933</cites><orcidid>0000-0003-4434-8351 ; 0000-0002-5212-3649</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3121049847/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3121049847?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,74998</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39461978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Jiaxi</creatorcontrib><creatorcontrib>Lammers, Nicholas C.</creatorcontrib><creatorcontrib>Alamos, Simon</creatorcontrib><creatorcontrib>Kim, Yang Joon</creatorcontrib><creatorcontrib>Martini, Gabriella</creatorcontrib><creatorcontrib>Garcia, Hernan G.</creatorcontrib><title>Optogenetic dissection of transcriptional repression in a multicellular organism</title><title>Nature communications</title><addtitle>Nat Commun</addtitle><addtitle>Nat Commun</addtitle><description>Transcriptional control is fundamental to cellular function. However, despite knowing that transcription factors can repress or activate specific genes, how these functions are implemented at the molecular level has remained elusive, particularly in the endogenous context of developing animals. Here, we combine optogenetics, single-cell live-imaging, and mathematical modeling to study how a zinc-finger repressor, Knirps, induces switch-like transitions into long-lived quiescent states. Using optogenetics, we demonstrate that repression is rapidly reversible (~1 min) and memoryless. Furthermore, we show that the repressor acts by decreasing the frequency of transcriptional bursts in a manner consistent with an equilibrium binding model. Our results provide a quantitative framework for dissecting the in vivo biochemistry of eukaryotic transcriptional regulation. Using optogenetics in the fly embryo, this study investigates how a transcriptional repressor drives switch-like, rapidly reversible repression by modulating transcriptional burst frequency, offering insights into gene regulation dynamics in development.</description><subject>631/136</subject><subject>631/1647/2253</subject><subject>631/553</subject><subject>631/57</subject><subject>Animals</subject><subject>Biology</subject><subject>Dissection</subject><subject>Embryos</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene loci</subject><subject>Gene regulation</subject><subject>Gene silencing</subject><subject>Genetics</subject><subject>Humanities and Social Sciences</subject><subject>In vivo methods and tests</subject><subject>Information processing</subject><subject>Mathematical models</subject><subject>multidisciplinary</subject><subject>Optics</subject><subject>Optogenetics</subject><subject>Proteins</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Single-Cell Analysis</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Zinc finger proteins</subject><subject>Zinc Fingers</subject><issn>2041-1723</issn><issn>2041-1723</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kk1v1DAQhiMEolXbP9ADisSFS8BjO7F9QqgCWqlSe6Bny2uPF6-SONgJUv893mZbWg74YmvmmXc-PFV1DuQjECY_ZQ68Ew2hvGlZy1RDXlXHlHBoQFD2-tn7qDrLeUfKYQok52-rI6Z4B0rI4-r2ZprjFkecg61dyBntHOJYR1_PyYzZpjDtDaavE04Jc957w1ibelj6EoR9v_Qm1TFtzRjycFq98abPeHa4T6q7b19_XFw21zffry6-XDeWCTk3XiJ1DlEwSalHAogbJ8EroJ0QaKhE39oNp7zthASOqrPMUudpC2gUYyfV1arrotnpKYXBpHsdTdAPhlKONqnU16PeMAfKO9Y5QbhBqjqFpGRoPQgBUhWtz6vWtGwGdBbH0nv_QvSlZww_9Tb-1gAtMKBtUfhwUEjx14J51kPI-9mYEeOSdYGAdFwRXtD3_6C7uKQy4APFleSiUHSlbIo5J_RP1QDR-wXQ6wLosgD6YQE0KUHvnvfxFPL43QVgK5CLa9xi-pv7P7J_ADiwvIo</recordid><startdate>20241026</startdate><enddate>20241026</enddate><creator>Zhao, Jiaxi</creator><creator>Lammers, Nicholas C.</creator><creator>Alamos, Simon</creator><creator>Kim, Yang Joon</creator><creator>Martini, Gabriella</creator><creator>Garcia, Hernan G.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-4434-8351</orcidid><orcidid>https://orcid.org/0000-0002-5212-3649</orcidid></search><sort><creationdate>20241026</creationdate><title>Optogenetic dissection of transcriptional repression in a multicellular organism</title><author>Zhao, Jiaxi ; Lammers, Nicholas C. ; Alamos, Simon ; Kim, Yang Joon ; Martini, Gabriella ; Garcia, Hernan G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-f8e2ddee73822fe01eebd81f912677ea28ef5cb424567814e96c3c2df251ea933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>631/136</topic><topic>631/1647/2253</topic><topic>631/553</topic><topic>631/57</topic><topic>Animals</topic><topic>Biology</topic><topic>Dissection</topic><topic>Embryos</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gene loci</topic><topic>Gene regulation</topic><topic>Gene silencing</topic><topic>Genetics</topic><topic>Humanities and Social Sciences</topic><topic>In vivo methods and tests</topic><topic>Information processing</topic><topic>Mathematical models</topic><topic>multidisciplinary</topic><topic>Optics</topic><topic>Optogenetics</topic><topic>Proteins</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Single-Cell Analysis</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Zinc finger proteins</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Jiaxi</creatorcontrib><creatorcontrib>Lammers, Nicholas C.</creatorcontrib><creatorcontrib>Alamos, Simon</creatorcontrib><creatorcontrib>Kim, Yang Joon</creatorcontrib><creatorcontrib>Martini, Gabriella</creatorcontrib><creatorcontrib>Garcia, Hernan G.</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health &amp; Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Nature communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Jiaxi</au><au>Lammers, Nicholas C.</au><au>Alamos, Simon</au><au>Kim, Yang Joon</au><au>Martini, Gabriella</au><au>Garcia, Hernan G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optogenetic dissection of transcriptional repression in a multicellular organism</atitle><jtitle>Nature communications</jtitle><stitle>Nat Commun</stitle><addtitle>Nat Commun</addtitle><date>2024-10-26</date><risdate>2024</risdate><volume>15</volume><issue>1</issue><spage>9263</spage><epage>11</epage><pages>9263-11</pages><artnum>9263</artnum><issn>2041-1723</issn><eissn>2041-1723</eissn><abstract>Transcriptional control is fundamental to cellular function. However, despite knowing that transcription factors can repress or activate specific genes, how these functions are implemented at the molecular level has remained elusive, particularly in the endogenous context of developing animals. Here, we combine optogenetics, single-cell live-imaging, and mathematical modeling to study how a zinc-finger repressor, Knirps, induces switch-like transitions into long-lived quiescent states. Using optogenetics, we demonstrate that repression is rapidly reversible (~1 min) and memoryless. Furthermore, we show that the repressor acts by decreasing the frequency of transcriptional bursts in a manner consistent with an equilibrium binding model. Our results provide a quantitative framework for dissecting the in vivo biochemistry of eukaryotic transcriptional regulation. Using optogenetics in the fly embryo, this study investigates how a transcriptional repressor drives switch-like, rapidly reversible repression by modulating transcriptional burst frequency, offering insights into gene regulation dynamics in development.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>39461978</pmid><doi>10.1038/s41467-024-53539-0</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4434-8351</orcidid><orcidid>https://orcid.org/0000-0002-5212-3649</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2041-1723
ispartof Nature communications, 2024-10, Vol.15 (1), p.9263-11, Article 9263
issn 2041-1723
2041-1723
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_b3d19fd36d704ae2969e0a285f177189
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central (Open access); Nature; Springer Nature - nature.com Journals - Fully Open Access
subjects 631/136
631/1647/2253
631/553
631/57
Animals
Biology
Dissection
Embryos
Gene expression
Gene Expression Regulation
Gene loci
Gene regulation
Gene silencing
Genetics
Humanities and Social Sciences
In vivo methods and tests
Information processing
Mathematical models
multidisciplinary
Optics
Optogenetics
Proteins
Repressor Proteins - genetics
Repressor Proteins - metabolism
Science
Science (multidisciplinary)
Single-Cell Analysis
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
Zinc finger proteins
Zinc Fingers
title Optogenetic dissection of transcriptional repression in a multicellular organism
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T21%3A25%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optogenetic%20dissection%20of%20transcriptional%20repression%20in%20a%20multicellular%20organism&rft.jtitle=Nature%20communications&rft.au=Zhao,%20Jiaxi&rft.date=2024-10-26&rft.volume=15&rft.issue=1&rft.spage=9263&rft.epage=11&rft.pages=9263-11&rft.artnum=9263&rft.issn=2041-1723&rft.eissn=2041-1723&rft_id=info:doi/10.1038/s41467-024-53539-0&rft_dat=%3Cproquest_doaj_%3E3121049847%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c378t-f8e2ddee73822fe01eebd81f912677ea28ef5cb424567814e96c3c2df251ea933%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3121049847&rft_id=info:pmid/39461978&rfr_iscdi=true