Generation of a PDGFRB-mCherry knock-in reporter human induced pluripotent stem cell line (KITi001-A-1), using CRISPR/Cas9 nuclease

PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-β), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-β...

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Bibliographic Details
Published in:Stem cell research 2023-06, Vol.69, p.103081-103081, Article 103081
Main Authors: Jo, Seongyea, Kim, Ji-Woo, Noh, Haneul, Kim, Hyemin, Kim, Jong-Hoon, Park, Han-Jin
Format: Article
Language:English
Subjects:
Cell Differentiation
Cell Line
CRISPR-Cas Systems - genetics
Humans
Induced Pluripotent Stem Cells - metabolism
Platelet-Derived Growth Factor - metabolism
Receptor, Platelet-Derived Growth Factor beta - genetics
Receptor, Platelet-Derived Growth Factor beta - metabolism
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Summary:PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-β), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-β plays an essential role in early liver diseases, including liver fibrosis. Here, we generated a human induced pluripotent stem cell (iPSC) line, KITi001-A-1, using CRISPR/Cas9. This reporter iPSC line and its derivatives are useful for tracing PDGFR-β-expressing cells and for screening for liver fibrosis-inducing compounds.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2023.103081