Polymorphisms associated to bovine paratuberculosis: investigation of their role in DNA-protein interactions and transcriptional regulation

Previous studies led to identify SNPs in putative regulatory regions of the SLC11A1 and CARD15 genes with association to paratuberculosis in cattle. Aim of this study was to investigate the role of these mutations at the regulatory level by DNA-protein interaction analyses and transcriptome comparis...

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Bibliographic Details
Published in:Veterinaria italiana 2020-12, Vol.56 (2)
Main Authors: Berik Aryngaziyev, Beltramo, Chiara, Dondo, Alessandro, Karymsakov, Talgat, Varello, Katia, Goria, Maria, Alessia Di Blasio, Nodari, Sabrina, Colussi, Silvia, Modesto, Paola, Daugaliyeva, Aida, Acutis, Pier Luigi, Saule Daugaliyeva, Peletto, Simone
Format: Article
Language:English
Subjects:
Alleles
Binding sites
Deoxyribonucleic acid
DNA
Electrophoretic mobility
EMSA
Gene regulation
Genes
Genomes
Immune system
Innate immunity
Paratubercolosis
Paratuberculosis
Protein interaction
Regulatory sequences
RNA-Seq
Single-nucleotide polymorphism
SNP
Transcription factors
Transcriptomes
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Summary:Previous studies led to identify SNPs in putative regulatory regions of the SLC11A1 and CARD15 genes with association to paratuberculosis in cattle. Aim of this study was to investigate the role of these mutations at the regulatory level by DNA-protein interaction analyses and transcriptome comparison between wild-type and mutated animals. Gene regions carrying the SNPs of interest were analysed by bioinformatic tools to predict allele-dependent binding sites for transcription factors (TFBS). Putative TFBS were in vitro explored by Electrophoretic Mobility Shift Assays (EMSA). EMSA did not show specific gel shifts for any allele indicating that these SNPs may eventually influence gene transcription without altering TFBS. Whole transcriptome expression analysis was performed on intestinal tissues of wild-type and mutated cattle by RNA-Seq. Differential regulation of five genes involved in innate immune system was detected. Specifically, ULBP3 was down-regulated, while S100A8, S100A12, LOC510860, and IFI27 were up-regulated. In previous studies, ULBP3, S100A8, and S100A12 resulted differentially expressed in cattle affected by paratuberculosis, suggesting a possible implication in the pathogen response. Further investigations are needed to elucidate the functional role of these SNPs and to understand the gene network involved in the interactions between non-coding SNPs and other genome regions.
ISSN:0505-401X
1828-1427
DOI:10.12834/VetIt.2325.13205.1