Differential proteomic analysis of nuclear matrix in muscle-invasive bladder cancer: potential to improve diagnosis and prognosis

Although several molecular markers for bladder cancer have been identified, at present little information on prognostic biomarkers is available in the literature. Prognostication of this tumor is largely based on clinicopathological characteristics. Our aim was to identify nuclear matrix (NM) protei...

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Published in:Analytical cellular pathology (Amsterdam) 2008, Vol.30 (1), p.13-26
Main Authors: Barboro, Paola, Rubagotti, Alessandra, Orecchia, Paola, Spina, Bruno, Truini, Mauro, Repaci, Erica, Carmignani, Giorgio, Romagnoli, Andrea, Introini, Carlo, Boccardo, Francesco, Carnemolla, Barbara, Balbi, Cecilia
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biomarkers, Tumor - analysis
Blotting, Western
Electrophoresis, Gel, Two-Dimensional
Female
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Neoplasm Proteins - analysis
Nuclear Matrix-Associated Proteins - analysis
Other
Prognosis
Proteome - analysis
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Urinary Bladder Neoplasms - chemistry
Urinary Bladder Neoplasms - diagnosis
Urinary Bladder Neoplasms - pathology
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Summary:Although several molecular markers for bladder cancer have been identified, at present little information on prognostic biomarkers is available in the literature. Prognostication of this tumor is largely based on clinicopathological characteristics. Our aim was to identify nuclear matrix (NM) proteins that might serve to better characterize the phenotype of the invasive bladder cancer and to investigate their diagnostic and prognostic roles. NM proteins expressed in normal (n=3) or non-tumoral (n=9) tissue specimens and muscle-invasive bladder cancer (n=21) specimens were analyzed by two dimensional (2D) gel electrophoresis. PDQuest image analysis software was used to generate a comparative NM proteome analysis. Selected spots were characterized by liquid chromatography coupled to tandem mass spectrometry and Western blot. We detected over 800 protein spots in each 2D map and 43 spots were identified. 30 proteins were differentially expressed by bladder tumor cells; among these, 19 proteins were detected in bladder tumoral tissues but not in normal and non-tumoral tissues and seven proteins correlated with tumor stage. One protein (p54nrb) was strongly correlated with vascular invasions and appeared to be also significantly (P
ISSN:1570-5870
2210-7177
2210-7185
1875-8606
DOI:10.1155/2008/686940