Ekspresi level gen mRNA protein ekstraseluler otak embrio mencit black-6 uk-12 akibat induksi 2-methoxyethanol : analisis secara real time RT-PCR

The aim of this research was to investigate impact of 2-methoxyethanol, a major industrial chemical, and its individual metaboliteson the expression DNA of the embryonic brain development of black-6 mice. The expression levels mRNA protein of GAPDH, Fibronectin,tenascin, vimentin, Neurofilamen, NCam...

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Bibliographic Details
Published in:Berkala Penelitian Hayati (Journal of Biological Researchers) 2012-02, Vol.15 (2), p.171-179
Main Authors: Yulia Irnidayanti, Win Darmanto, Agus Abadi
Format: Article
Language:English
Subjects:
2-methoxyethanol
Fibronectin
GAPDH
Ncam
Neurofilamen
Otak
Tenascin
Vimentin
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Summary:The aim of this research was to investigate impact of 2-methoxyethanol, a major industrial chemical, and its individual metaboliteson the expression DNA of the embryonic brain development of black-6 mice. The expression levels mRNA protein of GAPDH, Fibronectin,tenascin, vimentin, Neurofilamen, NCam between brain embrio treatment with 2-ME at gestation day 12 and Embryo control wereachieved. The Electroforesis DNA on brain Embryonic day 12 showed that there were expression of GAPDH (447bp), Fibronectin(462bp), NCAM (293 bp), Tenascin (416bp), Vimentin (327), Neurofilamen high (301bp), Neurofilamen medium (289bp), Neurofilamenlow (398bp). This Data not showed. The expression of level of mRNA for protein Vimentin at embryonic brain treatment at GD-12 is487 copies, meanwhile on the embryinoc brain control is 209 copies. This expression is tendency very higher than control. Anotherlevel of mRNA for protein fibronectin, NCAM, Tenascin, Neurofilament were tendency not differe between embryinoc brain treatmentsand control. Intermediate filaments, vimentin, is found in specific cell types in the developing and adult central nervous systems (CNS),particularly astrocytes. Recently, found that vimentin immunoreactivities were increased in astrocytes and/or macrophages in the spinalcords of rats with autoimmune inflammation). So that The higher level mRNA for protein vimentin caused by effect 2-methoxyethanol.Vimentin contribute to the repair of brain through the migration of activated cells and increased level vimentin at embryionic braintreatment with 2-ME.
ISSN:0852-6834
2337-389X
DOI:10.23869/bphjbr.15.2.201011