Experimental Model of Human Malignant Mesothelioma in Athymic Mice

Malignant pleural mesothelioma (MPM) is a thoracic aggressive cancer caused by asbestos exposure, which is difficult to diagnose and treat. Here, we characterized an in vivo orthotopic xenograft model consisting of human mesothelioma cells (designed as H2052/484) derived from a pleural NCI-H2052 tum...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-06, Vol.19 (7), p.1881
Main Authors: Colin, Didier J, Cottet-Dumoulin, David, Faivre, Anna, Germain, Stéphane, Triponez, Frédéric, Serre-Beinier, Véronique
Format: Article
Language:English
Subjects:
Animals
Asbestos
athymic mouse
Body Fluids - metabolism
Cancer
Carcinogenesis - pathology
Cell Count
Cell Line, Tumor
Cell Survival
Epithelial-Mesenchymal Transition - genetics
Gene Expression Regulation, Neoplastic
Humans
immune cells
Immune system
Immunodeficiency
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - immunology
Lung Neoplasms - pathology
Macrophages
Macrophages - metabolism
Mesothelioma
Mesothelioma - genetics
Mesothelioma - immunology
Mesothelioma - pathology
Mesothelioma, Malignant
Mice, Nude
orthotopic xenotransplantation
Phenotypes
pleura
Pleural fluid
Thorax
Tumors
Xenograft Model Antitumor Assays
Xenografts
Xenotransplantation
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Summary:Malignant pleural mesothelioma (MPM) is a thoracic aggressive cancer caused by asbestos exposure, which is difficult to diagnose and treat. Here, we characterized an in vivo orthotopic xenograft model consisting of human mesothelioma cells (designed as H2052/484) derived from a pleural NCI-H2052 tumor injected in partially immunodeficient athymic mice. We assessed tumor formation and tumor-dependent patterns of inflammation. H2052/484 cells conserved their mesothelioma phenotype and most characteristics from the parental NCI-H2052 cells. After intra-thoracic injection of H2052/484 cells, thoracic tumors developed in nearly all mice (86%) within 14 days, faster than from parental NCI-H2052 cells. When the mice were euthanized, the pleural lavage fluid was examined for immune cell profiles. The pleural immune cell population increased with tumor development. Interestingly, the proportion of myeloid-derived suppressor cell and macrophage (especially CD206⁺ M2 macrophages) populations increased in the pleural fluid of mice with large mesothelioma development, as previously observed in immunocompetent mice. This reliable orthotopic model recapitulates human mesothelioma and may be used for the study of new treatment strategies.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19071881