Physiological and Genetic Modifications Induced by Plant-Growth-Promoting Rhizobacteria (PGPR) in Tomato Plants under Moderate Water Stress

• Published in: Biology (Basel, Switzerland) Switzerland), 2023-06, Vol.12 (7), p.901
• Main Authors: Lucas, Jose Antonio, Garcia-Villaraco, Ana, Montero-Palmero, Maria Belen, Montalban, Blanca, Ramos Solano, Beatriz, Gutierrez-Mañero, Francisco Javier
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Adaptation; Adenosine triphosphatase; Agriculture; Aquatic resources; Ascorbic acid; Bacteria; Carbon fixation; Carotenoids; Cell division; Chlorophyll; Climate change; Climatic changes; Defensive behavior; Dioxygenase; Drinking water; Efficiency; Enzymes; Food consumption; Food security; Food supply; Gene expression; Genetic engineering; Genetically modified organisms; Glycine betaine; Growth; Guaiacol; L-Ascorbate peroxidase; Metabolism; Oxidative stress; P5CS; PEG6000; PGPR; Phenolic compounds; Photosynthesis; photosynthesis efficiency; Photosynthetic pigments; Physiology; Pigments; Plant physiology; Polyethylene glycol; Reactive oxygen species; Spain; Stomata; Strains (organisms); Tomatoes; Transpiration; Water shortages; Water stress
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology12070901

Physiological, metabolic, and genetic changes produced by two plant growth promoting rhizobacteria (PGPR) sp. (internal code of the laboratory: N 5.12 and N 21.24) inoculated in tomato plants subjected to moderate water stress (10% polyethylene glycol-6000; PEG) were studied. Photosynthesis efficiency, photosynthetic pigments, compatible osmolytes, reactive oxygen species (ROS) scavenging enzymes activities, oxidative stress level and expression of genes related to abscisic acid synthesis (ABA; 9-cis-epoxycarotenoid dioxygenase gene), proline synthesis (Pyrroline-5-carboxylate synthase gene), and plasma membrane ATPase ( gene) were measured. Photosynthetic efficiency was compromised by PEG, but bacterial-inoculated plants reversed the effects: while N5.12 increased carbon fixation (37.5%) maintaining transpiration, N21.24 increased both (14.2% and 31%), negatively affecting stomatal closure, despite the enhanced expression of NCDE1 and plasma membrane ATPase genes, evidencing the activation of different adaptive mechanisms. Among all parameters evaluated, photosynthetic pigments and antioxidant enzymes guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) responded differently to both strains. N 5.12 increased photosynthetic pigments (70% chlorophyll a, 69% chlorophyll b, and 65% carotenoids), proline (33%), glycine betaine (4.3%), and phenolic compounds (21.5%) to a greater extent, thereby decreasing oxidative stress (12.5% in Malondialdehyde, MDA). Both bacteria have highly beneficial effects on tomato plants subjected to moderate water stress, improving their physiological state. The use of these bacteria in agricultural production systems could reduce the amount of water for agricultural irrigation without having a negative impact on food production.