Total antioxidant capacity in dogs with gastric dilatation and volvulus

• Published in: Veterinární medicína 2012-04, Vol.57 (4), p.207-211
• Main Authors: Uhrikova, I., Rehakova, K., Rauserova-Lexmaulova, L., Lojek, A., Doubek, J.
• Format: Article
• Language: English
• Subjects: Antioxidants; Correlation analysis; Dogs; gastric dilatation-volvulus syndrome (gdv); high mobility group box 1 protein (hmgb1); High mobility group proteins; HMGB1 protein; lactate; Lactic acid; Parameters; Reperfusion; Stretching; total radical-trapping antioxidant parameter (trap)
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/5918-VETMED

The aim of this study was to determine total antioxidant capacity in dogs with gastric dilatation-volvulus syndrome (GDV) and its correlations with high mobility group box 1 protein (HMGB1) and lactate concentrations. Correlation analyses between the measured parameters and disease severity were also performed. Fourteen dogs with GDV and six control dogs were used in this study. Blood was collected at the time of admission and again in the early reperfusion period. To assess antioxidant capacity, total radical-trapping antioxidant parameter (TRAP) analysis was performed. No significant difference in TRAP values existed between healthy dogs and dogs with GDV at admission. In the reperfusion period, TRAP values decreased in six dogs and increased in eight dogs. Changes in TRAP values strongly correlated with HMGB1 values (r = −0.83, P < 0.01) in the reperfusion period. Strong correlations between disease severity and TRAP values, HMGB1 and lactate levels were also found.