Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans

Ethyl carbamate is a common contaminant prevalent in fermented food with probable carcinogenic effects in animals. To date, other toxicological properties of ethyl carbamate are not well characterized. Using the genetic model Caenorhabditis elegans, we found that chronic exposure to ethyl carbamate...

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Bibliographic Details
Published in:Toxicology reports 2022-01, Vol.9, p.619-627
Main Authors: Comfort, Jordan J., Chomyshen, Samantha C., Waddell, Brandon M., Tabarraei, Hadi, Wu, Cheng-Wei
Format: Article
Language:English
Subjects:
adulthood
antioxidants
C. elegans
Caenorhabditis elegans
carcinogenicity
chronic exposure
ethyl carbamate
fermented foods
gene expression
gene expression regulation
genetic models
larval development
longevity
neurons
Neurotoxicity
Oxidative stress
Regular
reproduction
sequence analysis
toxicology
transcriptome
transcriptomics
Xenobiotics
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Summary:Ethyl carbamate is a common contaminant prevalent in fermented food with probable carcinogenic effects in animals. To date, other toxicological properties of ethyl carbamate are not well characterized. Using the genetic model Caenorhabditis elegans, we found that chronic exposure to ethyl carbamate during larval development impedes growth while exposure during adulthood inhibits reproduction, shortens lifespan, and promotes degeneration to dopaminergic neurons. Through whole-transcriptome RNA-sequencing, we found that ethyl carbamate invokes a widespread transcriptomic response inducing the differential expression of > 4,000 genes by at least 2-fold. Functional analysis of RNA-sequencing data revealed that up-regulated genes enrich to various neuron regulatory processes and xenobiotic defense. Gene expression analysis confirms that various genes encoding antioxidant enzymes and those functioning within phase I and II detoxification responses along with ABC transporters are highly up-regulated after ethyl carbamate exposure, suggesting the onset of oxidative stress. Overall, these findings report new toxicological properties of chronic ethyl carbamate exposure and provide new insights on its effects on transcriptome regulation in the C. elegans model. [Display omitted] •Ethyl carbamate is toxic towards development, reproduction, and aging in C. elegans.•Dopaminergic neurodegeneration is induced after prolonged ethyl carbamate exposure.•C. elegans respond to ethyl carbamate by activating xenobiotic detoxification.
ISSN:2214-7500
2214-7500
DOI:10.1016/j.toxrep.2022.03.029