The effect of potential-dependent potassium uptake on membrane potential in rat brain mitochondria

The effect of potential-dependent potassium uptake on the transmembrane potential difference (ΔΨm) in rat brain mitochondria has been studied­. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K+-uptake into matrix leads to the increase in respiration rate and m...

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Bibliographic Details
Published in:Ukrainian biochemical journal 2013-02, Vol.85 (1), p.33-41
Main Author: Akopova, O. V.
Format: Article
Language:English
Subjects:
brain mitochondria
K(+)
K+ATP-channel
membrane potential
oxygen consumption
transport
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Summary:The effect of potential-dependent potassium uptake on the transmembrane potential difference (ΔΨm) in rat brain mitochondria has been studied­. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K+-uptake into matrix leads to the increase in respiration rate and mitochondrial depolarization. ATP-dependent potassium channel (K+ATP-channel) blockers, gliben­clamide and 5-hydroxydecanoate, block ~35% of potential-dependent potassium uptake in the brain mitochondria. It was shown that K+ATP-channel blockage results in membrane repolarization by ~20% of control, which is consistent with experimental dependence of ΔΨm on the rate of potential-dependent potassium uptake. Obtained experimental data give the evidence that functional activity of K+ATP-channel is physiologically important in the regulation of membrane potential and energy-dependent processes in brain mitochondria.
ISSN:2409-4943
2413-5003
DOI:10.15407/ubj85.01.033