Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex

Atomic-resolution structure determination is crucial for understanding protein function. Cryo-EM and NMR spectroscopy both provide structural information, but currently cryo-EM does not routinely give access to atomic-level structural data, and, generally, NMR structure determination is restricted t...

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Bibliographic Details
Published in:Nature communications 2019-06, Vol.10 (1), p.2697-12, Article 2697
Main Authors: Gauto, Diego F., Estrozi, Leandro F., Schwieters, Charles D., Effantin, Gregory, Macek, Pavel, Sounier, Remy, Sivertsen, Astrid C., Schmidt, Elena, Kerfah, Rime, Mas, Guillaume, Colletier, Jacques-Philippe, Güntert, Peter, Favier, Adrien, Schoehn, Guy, Schanda, Paul, Boisbouvier, Jerome
Format: Article
Language:English
Subjects:
101/6
140/131
147/28
631/45/535/1258/1259
631/535/878
631/535/878/1264
Amides
Aminopeptidase
Aminopeptidases - chemistry
Aminopeptidases - ultrastructure
Atomic structure
Bacterial Proteins - chemistry
Bacterial Proteins - ultrastructure
Biochemistry, Molecular Biology
Biophysics
Cryoelectron Microscopy - methods
Crystallography
Enzymes
Humanities and Social Sciences
Laboratories
Life Sciences
Magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy - methods
Molecular Dynamics Simulation
Molecular structure
Molecular Weight
multidisciplinary
Multienzyme Complexes - chemistry
Multienzyme Complexes - ultrastructure
NMR
NMR spectroscopy
Nuclear magnetic resonance
Protein Structure, Quaternary
Proteins
Pyrococcus horikoshii
Science
Science (multidisciplinary)
Structural Biology
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Description
Summary:Atomic-resolution structure determination is crucial for understanding protein function. Cryo-EM and NMR spectroscopy both provide structural information, but currently cryo-EM does not routinely give access to atomic-level structural data, and, generally, NMR structure determination is restricted to small (
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-019-10490-9