Antibiofilm and Antihemolytic Activities of Actinostemma lobatum Extract Rich in Quercetin against Staphylococcus aureus

biofilm formation is a pivotal mechanism in the development of drug resistance, conferring resilience against conventional antibiotics. This study investigates the inhibitory effects of ( ) Maxim extracts on biofilm formation and their antihemolytic activities, with a particular focus on identifying...

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Bibliographic Details
Published in:Pharmaceutics 2024-08, Vol.16 (8), p.1075
Main Authors: Lee, Jin-Hyung, Kim, Yong-Guy, Choi, Ji-Su, Jeong, Yong Tae, Hwang, Buyng Su, Lee, Jintae
Format: Article
Language:English
Subjects:
Actinostemma lobatum
antibiofilm
Antibiotics
antihemolysis
Biofilms
Cell growth
Electron microscopes
Ethanol
NMR
Nosocomial infections
Nuclear magnetic resonance
Pathogens
quercetin
Scanning electron microscopy
Solvents
Staphylococcus aureus
Staphylococcus infections
Toxins
Virulence
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Summary:biofilm formation is a pivotal mechanism in the development of drug resistance, conferring resilience against conventional antibiotics. This study investigates the inhibitory effects of ( ) Maxim extracts on biofilm formation and their antihemolytic activities, with a particular focus on identifying the active antibiofilm and antihemolysis compound, quercetin. Seven solvent extracts and twelve sub-fractions were evaluated against four strains. The ethyl acetate fraction (10 to 100 μg/mL) significantly hindered biofilm formation by both methicillin-sensitive and -resistant strains. Bioassay-guided isolation of the ethyl acetate extract identified quercetin as the major antibiofilm compound. The ethyl acetate extract was found to contain 391 μg/mg of quercetin and 30 μg/mg of kaempferol. Additionally, the extract exhibited antihemolytic activity attributable to the presence of quercetin. The findings suggest that quercetin-rich extracts from and other quercetin-rich foods and plants hold promise for inhibiting resilient biofilm formation and attenuating its virulence.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics16081075