In vitro Suppression of SARS-CoV-2 Infection by Existing Kampo Formulas and Crude Constituent Drugs Used for Treatment of Common Cold Respiratory Symptoms

Several traditional Japanese Kampo formulas are known to have inhibitory effects on infections with viruses that cause respiratory symptoms. Although some herbs and their components have been reported to suppress SARS-CoV-2 replication , it is difficult to compare effective Kampo formulas because of...

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Bibliographic Details
Published in:Frontiers in pharmacology 2022-03, Vol.13, p.804103-804103
Main Authors: Kakimoto, Masaki, Nomura, Toshihito, Nazmul, Tanuza, Kitagawa, Hiroki, Kanno, Keishi, Ogawa-Ochiai, Keiko, Ohge, Hiroki, Ito, Masanori, Sakaguchi, Takemasa
Format: Article
Language:English
Subjects:
anti-viral
COVID-19
crude drug
herbal medicine
kampo medicine
Pharmacology
SARS-CoV-2
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Summary:Several traditional Japanese Kampo formulas are known to have inhibitory effects on infections with viruses that cause respiratory symptoms. Although some herbs and their components have been reported to suppress SARS-CoV-2 replication , it is difficult to compare effective Kampo formulas because of the different methods used in studies. Thus, we carried out experiments on the suppression of SARS-CoV-2 infection by Kampo formulas and crude drugs used for the common cold to compare their suppressive effects on virus infection. After infecting VeroE6/TMPRSS2 cells with SARS-CoV-2, lysates of the Kampo formulas and crude drugs were added, and after 24 h, the infectious titer in the medium was measured by the TCID method. Maoto was the most effective among the Kampo formulas, and Ephedrae herba was the most effective among the constituent crude drugs. However, a comparison of the suppressive effects of Ephedrae herba and Kampo formulas containing Ephedrae herba showed that the suppressive effect on virus infection did not depend on the content of Ephedrae herba. Based on the results, we believe that the use of Maoto among Kampo formulas is suitable as a countermeasure against COVID-19.
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2022.804103