Loading…
Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons
Accumulated evidence suggests that hydrogen peroxide (H2O2) generated in cells during insulin stimulation plays an integral role in insulin receptor signal transduction. The role of insulin-induced H2O2 in neuronal insulin receptor activation and the origin of insulin-induced H2O2 in neurons remain...
Saved in:
Published in: | BMC neuroscience 2007-10, Vol.8 (1), p.84-84, Article 84 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373 |
---|---|
cites | cdi_FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373 |
container_end_page | 84 |
container_issue | 1 |
container_start_page | 84 |
container_title | BMC neuroscience |
container_volume | 8 |
creator | Storozhevykh, Tatiana P Senilova, Yana E Persiyantseva, Nadezhda A Pinelis, Vsevolod G Pomytkin, Igor A |
description | Accumulated evidence suggests that hydrogen peroxide (H2O2) generated in cells during insulin stimulation plays an integral role in insulin receptor signal transduction. The role of insulin-induced H2O2 in neuronal insulin receptor activation and the origin of insulin-induced H2O2 in neurons remain unclear. The aim of the present study is to test the following hypotheses (1) whether insulin-induced H2O2 is required for insulin receptor autophosphorylation in neurons, and (2) whether mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in insulin receptor autophosphorylation in neurons.
Insulin stimulation elicited rapid insulin receptor autophosphorylation accompanied by an increase in H2O2 release from cultured cerebellar granule neurons (CGN). N-acetylcysteine (NAC), a H2O2 scavenger, inhibited both insulin-stimulated H2O2 release and insulin-stimulated autophosphorylation of insulin receptor. Inhibitors of respiratory chain-mediated H2O2 production, malonate and carbonyl cyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP), inhibited both insulin-stimulated H2O2 release from neurons and insulin-stimulated autophosphorylation of insulin receptor. Dicholine salt of succinic acid, a respiratory substrate, significantly enhanced the effect of suboptimal insulin concentration on the insulin receptor autophosphorylation in CGN.
Results of the present study suggest that insulin-induced H2O2 is required for the enhancement of insulin receptor autophosphorylation in neurons. The mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in the insulin receptor autophosphorylation in neurons. |
doi_str_mv | 10.1186/1471-2202-8-84 |
format | article |
fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_b47c4e02f849468cbd5aaf183c5accd7</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A171640464</galeid><doaj_id>oai_doaj_org_article_b47c4e02f849468cbd5aaf183c5accd7</doaj_id><sourcerecordid>A171640464</sourcerecordid><originalsourceid>FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373</originalsourceid><addsrcrecordid>eNp1ksFu1DAQhiMEoqVw5YgiIXFLsWMncS5Iq4pCpSIucLYce7LrKvEE21l134mHxOmuyq4osiyPZ35_M2M7y95SckmpqD9S3tCiLElZiELwZ9n5o-P5kX2WvQrhjhDaCF6-zM5o09KWcXae_f5mI-oNOuOtGnIPYbJeRfS7XG-UdbkNuXVbHLZg8mXrwjxYV4Rox3lQMXk3O-NxDS6fwOO9NZBPHs2so0WXK2fyaVC7kKx0OMLaL2lwgCNaSqthSklzNUecNhjS9LuEXxAp7mD26MLr7EWvhgBvDutF9vP684-rr8Xt9y83V6vboqubOhataDved6znrANdV6RqiVKalj0nqe--N5WuW94IRhnQRpec1YI3Fas7ToE17CK72XMNqjs5eTsqv5OorHxwoF9L5aPVA8iON5oDKXvBW14L3ZlKqZ4KpiultVlYn_asae5GMBpcTDdwAj2NOLuRa9zKkoiWNAtgtQd0Fv8DOI1oHOXy8nJ5eSmk4Inx4VCEx18zhChHGzQMg3KAc0i5GBe8qpPw_V64Vqk563pMSL2I5Yo2tOaE1wvu8glVGgZGq9FBb5P_qQPaYwge-sfyKZHLP_634HfHt_ZXfvi47A99K_N7</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20348456</pqid></control><display><type>article</type><title>Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons</title><source>PubMed Central</source><creator>Storozhevykh, Tatiana P ; Senilova, Yana E ; Persiyantseva, Nadezhda A ; Pinelis, Vsevolod G ; Pomytkin, Igor A</creator><creatorcontrib>Storozhevykh, Tatiana P ; Senilova, Yana E ; Persiyantseva, Nadezhda A ; Pinelis, Vsevolod G ; Pomytkin, Igor A</creatorcontrib><description>Accumulated evidence suggests that hydrogen peroxide (H2O2) generated in cells during insulin stimulation plays an integral role in insulin receptor signal transduction. The role of insulin-induced H2O2 in neuronal insulin receptor activation and the origin of insulin-induced H2O2 in neurons remain unclear. The aim of the present study is to test the following hypotheses (1) whether insulin-induced H2O2 is required for insulin receptor autophosphorylation in neurons, and (2) whether mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in insulin receptor autophosphorylation in neurons.
Insulin stimulation elicited rapid insulin receptor autophosphorylation accompanied by an increase in H2O2 release from cultured cerebellar granule neurons (CGN). N-acetylcysteine (NAC), a H2O2 scavenger, inhibited both insulin-stimulated H2O2 release and insulin-stimulated autophosphorylation of insulin receptor. Inhibitors of respiratory chain-mediated H2O2 production, malonate and carbonyl cyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP), inhibited both insulin-stimulated H2O2 release from neurons and insulin-stimulated autophosphorylation of insulin receptor. Dicholine salt of succinic acid, a respiratory substrate, significantly enhanced the effect of suboptimal insulin concentration on the insulin receptor autophosphorylation in CGN.
Results of the present study suggest that insulin-induced H2O2 is required for the enhancement of insulin receptor autophosphorylation in neurons. The mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in the insulin receptor autophosphorylation in neurons.</description><identifier>ISSN: 1471-2202</identifier><identifier>EISSN: 1471-2202</identifier><identifier>DOI: 10.1186/1471-2202-8-84</identifier><identifier>PMID: 17919343</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Cell Respiration - physiology ; Cells, Cultured ; Electron Transport - physiology ; Health aspects ; Hydrogen Peroxide - metabolism ; Insulin ; Insulin - metabolism ; Insulin - pharmacology ; Insulin - physiology ; Krebs cycle ; Mitochondria - metabolism ; Neurons ; Neurons - metabolism ; Neurons - physiology ; Phosphorylation ; Rats ; Rats, Wistar ; Receptor, Insulin - metabolism ; Receptors</subject><ispartof>BMC neuroscience, 2007-10, Vol.8 (1), p.84-84, Article 84</ispartof><rights>COPYRIGHT 2007 BioMed Central Ltd.</rights><rights>Copyright © 2007 Storozhevykh et al.; licensee BioMed Central Ltd. 2007 Storozhevykh et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373</citedby><cites>FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2089077/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2089077/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17919343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Storozhevykh, Tatiana P</creatorcontrib><creatorcontrib>Senilova, Yana E</creatorcontrib><creatorcontrib>Persiyantseva, Nadezhda A</creatorcontrib><creatorcontrib>Pinelis, Vsevolod G</creatorcontrib><creatorcontrib>Pomytkin, Igor A</creatorcontrib><title>Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons</title><title>BMC neuroscience</title><addtitle>BMC Neurosci</addtitle><description>Accumulated evidence suggests that hydrogen peroxide (H2O2) generated in cells during insulin stimulation plays an integral role in insulin receptor signal transduction. The role of insulin-induced H2O2 in neuronal insulin receptor activation and the origin of insulin-induced H2O2 in neurons remain unclear. The aim of the present study is to test the following hypotheses (1) whether insulin-induced H2O2 is required for insulin receptor autophosphorylation in neurons, and (2) whether mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in insulin receptor autophosphorylation in neurons.
Insulin stimulation elicited rapid insulin receptor autophosphorylation accompanied by an increase in H2O2 release from cultured cerebellar granule neurons (CGN). N-acetylcysteine (NAC), a H2O2 scavenger, inhibited both insulin-stimulated H2O2 release and insulin-stimulated autophosphorylation of insulin receptor. Inhibitors of respiratory chain-mediated H2O2 production, malonate and carbonyl cyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP), inhibited both insulin-stimulated H2O2 release from neurons and insulin-stimulated autophosphorylation of insulin receptor. Dicholine salt of succinic acid, a respiratory substrate, significantly enhanced the effect of suboptimal insulin concentration on the insulin receptor autophosphorylation in CGN.
Results of the present study suggest that insulin-induced H2O2 is required for the enhancement of insulin receptor autophosphorylation in neurons. The mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in the insulin receptor autophosphorylation in neurons.</description><subject>Animals</subject><subject>Cell Respiration - physiology</subject><subject>Cells, Cultured</subject><subject>Electron Transport - physiology</subject><subject>Health aspects</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin - pharmacology</subject><subject>Insulin - physiology</subject><subject>Krebs cycle</subject><subject>Mitochondria - metabolism</subject><subject>Neurons</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Phosphorylation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor, Insulin - metabolism</subject><subject>Receptors</subject><issn>1471-2202</issn><issn>1471-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp1ksFu1DAQhiMEoqVw5YgiIXFLsWMncS5Iq4pCpSIucLYce7LrKvEE21l134mHxOmuyq4osiyPZ35_M2M7y95SckmpqD9S3tCiLElZiELwZ9n5o-P5kX2WvQrhjhDaCF6-zM5o09KWcXae_f5mI-oNOuOtGnIPYbJeRfS7XG-UdbkNuXVbHLZg8mXrwjxYV4Rox3lQMXk3O-NxDS6fwOO9NZBPHs2so0WXK2fyaVC7kKx0OMLaL2lwgCNaSqthSklzNUecNhjS9LuEXxAp7mD26MLr7EWvhgBvDutF9vP684-rr8Xt9y83V6vboqubOhataDved6znrANdV6RqiVKalj0nqe--N5WuW94IRhnQRpec1YI3Fas7ToE17CK72XMNqjs5eTsqv5OorHxwoF9L5aPVA8iON5oDKXvBW14L3ZlKqZ4KpiultVlYn_asae5GMBpcTDdwAj2NOLuRa9zKkoiWNAtgtQd0Fv8DOI1oHOXy8nJ5eSmk4Inx4VCEx18zhChHGzQMg3KAc0i5GBe8qpPw_V64Vqk563pMSL2I5Yo2tOaE1wvu8glVGgZGq9FBb5P_qQPaYwge-sfyKZHLP_634HfHt_ZXfvi47A99K_N7</recordid><startdate>20071008</startdate><enddate>20071008</enddate><creator>Storozhevykh, Tatiana P</creator><creator>Senilova, Yana E</creator><creator>Persiyantseva, Nadezhda A</creator><creator>Pinelis, Vsevolod G</creator><creator>Pomytkin, Igor A</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20071008</creationdate><title>Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons</title><author>Storozhevykh, Tatiana P ; Senilova, Yana E ; Persiyantseva, Nadezhda A ; Pinelis, Vsevolod G ; Pomytkin, Igor A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Cell Respiration - physiology</topic><topic>Cells, Cultured</topic><topic>Electron Transport - physiology</topic><topic>Health aspects</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Insulin</topic><topic>Insulin - metabolism</topic><topic>Insulin - pharmacology</topic><topic>Insulin - physiology</topic><topic>Krebs cycle</topic><topic>Mitochondria - metabolism</topic><topic>Neurons</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Phosphorylation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptor, Insulin - metabolism</topic><topic>Receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Storozhevykh, Tatiana P</creatorcontrib><creatorcontrib>Senilova, Yana E</creatorcontrib><creatorcontrib>Persiyantseva, Nadezhda A</creatorcontrib><creatorcontrib>Pinelis, Vsevolod G</creatorcontrib><creatorcontrib>Pomytkin, Igor A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>BMC neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Storozhevykh, Tatiana P</au><au>Senilova, Yana E</au><au>Persiyantseva, Nadezhda A</au><au>Pinelis, Vsevolod G</au><au>Pomytkin, Igor A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons</atitle><jtitle>BMC neuroscience</jtitle><addtitle>BMC Neurosci</addtitle><date>2007-10-08</date><risdate>2007</risdate><volume>8</volume><issue>1</issue><spage>84</spage><epage>84</epage><pages>84-84</pages><artnum>84</artnum><issn>1471-2202</issn><eissn>1471-2202</eissn><abstract>Accumulated evidence suggests that hydrogen peroxide (H2O2) generated in cells during insulin stimulation plays an integral role in insulin receptor signal transduction. The role of insulin-induced H2O2 in neuronal insulin receptor activation and the origin of insulin-induced H2O2 in neurons remain unclear. The aim of the present study is to test the following hypotheses (1) whether insulin-induced H2O2 is required for insulin receptor autophosphorylation in neurons, and (2) whether mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in insulin receptor autophosphorylation in neurons.
Insulin stimulation elicited rapid insulin receptor autophosphorylation accompanied by an increase in H2O2 release from cultured cerebellar granule neurons (CGN). N-acetylcysteine (NAC), a H2O2 scavenger, inhibited both insulin-stimulated H2O2 release and insulin-stimulated autophosphorylation of insulin receptor. Inhibitors of respiratory chain-mediated H2O2 production, malonate and carbonyl cyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP), inhibited both insulin-stimulated H2O2 release from neurons and insulin-stimulated autophosphorylation of insulin receptor. Dicholine salt of succinic acid, a respiratory substrate, significantly enhanced the effect of suboptimal insulin concentration on the insulin receptor autophosphorylation in CGN.
Results of the present study suggest that insulin-induced H2O2 is required for the enhancement of insulin receptor autophosphorylation in neurons. The mitochondrial respiratory chain is involved in insulin-stimulated H2O2 production, thus playing an integral role in the insulin receptor autophosphorylation in neurons.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>17919343</pmid><doi>10.1186/1471-2202-8-84</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1471-2202 |
ispartof | BMC neuroscience, 2007-10, Vol.8 (1), p.84-84, Article 84 |
issn | 1471-2202 1471-2202 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_b47c4e02f849468cbd5aaf183c5accd7 |
source | PubMed Central |
subjects | Animals Cell Respiration - physiology Cells, Cultured Electron Transport - physiology Health aspects Hydrogen Peroxide - metabolism Insulin Insulin - metabolism Insulin - pharmacology Insulin - physiology Krebs cycle Mitochondria - metabolism Neurons Neurons - metabolism Neurons - physiology Phosphorylation Rats Rats, Wistar Receptor, Insulin - metabolism Receptors |
title | Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A08%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mitochondrial%20respiratory%20chain%20is%20involved%20in%20insulin-stimulated%20hydrogen%20peroxide%20production%20and%20plays%20an%20integral%20role%20in%20insulin%20receptor%20autophosphorylation%20in%20neurons&rft.jtitle=BMC%20neuroscience&rft.au=Storozhevykh,%20Tatiana%20P&rft.date=2007-10-08&rft.volume=8&rft.issue=1&rft.spage=84&rft.epage=84&rft.pages=84-84&rft.artnum=84&rft.issn=1471-2202&rft.eissn=1471-2202&rft_id=info:doi/10.1186/1471-2202-8-84&rft_dat=%3Cgale_doaj_%3EA171640464%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b676t-989b4fb3f43bec650590aac12f40919ffd5c69478313e17c2436847536b41e373%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20348456&rft_id=info:pmid/17919343&rft_galeid=A171640464&rfr_iscdi=true |