LC–MS3 Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring

This study developed a detection method based on the strategy of HPLC/MS3 and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS3) system was performed using a Shimadzu UFLC XR liquid chromatography and...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-02, Vol.27 (4), p.1224
Main Authors: Ma, Dongxiao, Ji, Zhengchao, Cao, Haiwei, Huang, Jing, Zeng, Lei, Yin, Lei
Format: Article
Language:English
Subjects:
Acetonitrile
Biological products
Blood levels
Blood plasma
Carbamazepine
Epilepsy
Flow velocity
Formic acid
High performance liquid chromatography
Immunoassay
Ions
LC-MS3
Linearity
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Methods
Monitoring
Optimization
Plasma
Quadrupoles
Retention
Selectivity
Solvents
Telemedicine
Therapeutic drug monitoring
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Summary:This study developed a detection method based on the strategy of HPLC/MS3 and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS3) system was performed using a Shimadzu UFLC XR liquid chromatography and a SCIEX QTRAP® 5500 linear ion trap triple quadrupole mass spectrometer. The specific operation was as follows: the sample protein was firstly precipitated using methanol, then carbamazepine and carbamazepine-D2N15 were separated on an ACQUITY UPLC HSS T3 column using the gradient elution with solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile) at a flow rate of 0.25 mL/min. Each sample was run for 7 min. This method was validated for various parameters including accuracy, precision, selectivity, linearity, LLOQ, etc. Only 5 μL of sample plasma could obtain the result of LLOD 0.5 µg/mL. The intra-day and inter-day precision was
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27041224