Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress

• Published in: Plants (Basel) 2022-11, Vol.11 (22), p.3178
• Main Authors: Soorni, Jahad, Kazemitabar, Seyed Kamal, Kahrizi, Danial, Dehestani, Ali, Bagheri, Nadali, Kiss, Attila, Kovács, Péter Gergő, Papp, István, Mirmazloum, Iman
• Format: Article
• Language: English
• Subjects: Abiotic stress; Acclimation; Acclimatization; Acclimatization (Plants); Adaptation; Agricultural production; Analysis; Antioxidants; Biomarkers; Biotypes; C. sativa; Camelina; Catalase; CBF protein; Chromosomes; Cold; Cold acclimation; Cold tolerance; Cold treatment; Cold weather; Comparative analysis; Cruciferae; Cultivars; Electrolyte leakage; Environmental aspects; Enzymatic activity; Enzyme activity; Enzymes; Flowers & plants; Freezing; freezing tolerance; Frost; Gene expression; Genes; Genetic aspects; Genetic research; Genetic transcription; Genomes; Glutathione; Glutathione peroxidase; Hardiness; Identification and classification; Lipid peroxidation; Lipids; Oilseeds; Peroxidase; Peroxidation; Physiological aspects; Physiology; Phytochemicals; Plant tolerance; Plants; Proline; Proteins; Stress; Superoxide dismutase; Transcription factors
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11223178

Cold-acclimated and non-acclimated contrasting Camelina ( L.) biotypes were investigated for changes in stress-associated biomarkers, including antioxidant enzyme activity, lipid peroxidation, protein, and proline content. In addition, a well-known freezing tolerance pathway participant known as C-repeat/DRE-binding factors (CBFs), an inducer of CBF expression (ICE1), and a cold-regulated ( ) genes of the ICE-CBF-COR pathway were studied at the transcriptional level on the doubled-haploid (DH) lines. Freezing stress had significant effects on all studied parameters. The cold-acclimated DH34 (a freezing-tolerant line) showed an overall better performance under freezing stress than non-acclimated plants. The non-cold-acclimated DH08 (a frost-sensitive line) showed the highest electrolyte leakage after freezing stress. The highest activity of antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) was also detected in non-acclimated plants, whereas the cold-acclimated plants showed lower enzyme activities upon stress treatment. Cold acclimation had a significantly positive effect on the total protein and proline content of stressed plants. The qRT-PCR analysis revealed significant differences in the expression and cold-inducibility of , , and genes among the samples of different treatments. The highest expression of all CBF genes was recorded in the non-acclimated frost-tolerant biotype after freezing stress. Interestingly a significantly higher expression of was detected in cold-acclimated samples of both frost-sensitive and -tolerant biotypes after freezing stress. The presented results provide more insights into freezing tolerance mechanisms in the Camelina plant from both a biochemical point of view and the expression of the associated genes.