Plasmodium falciparum Merozoite Surface Protein-1 Polymorphisms among Asymptomatic Sickle Cell Anemia Patients in Nigeria

Asymptomatic malaria (ASM) has been implicated in the development of hemolytic crisis in infected sickle cell anemia (SCA) patients worldwide. This study surveyed steady state SCA Nigerian patients for ASM to investigate the influence of malaria prevention behaviors and age on parasitaemia and multi...

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Bibliographic Details
Published in:Acta medica Iranica 2016, Vol.54 (1), p.44-53
Main Authors: Bamidele Abiodun, Iwalokun, Oluwadun, Afolabi, Olugbenga Ayoola, Aina, Senapon Olusola, Iwalokun
Format: Article
Language:English
Subjects:
Adolescent
Adult
Alleles
Anemia, Sickle Cell - parasitology
Asymptomatic malaria
Chemoprophylaxis
Child
Child, Preschool
Cross-Sectional Studies
Female
Gene Frequency
Genotype
Humans
Malaria, Falciparum - parasitology
Male
Merozoite Surface Protein 1 - genetics
Multiplicity of infection
Nigeria
Parasite diversity
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - isolation & purification
Polymerase Chain Reaction
Polymorphism, Genetic
Prevalence
Young Adult
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Summary:Asymptomatic malaria (ASM) has been implicated in the development of hemolytic crisis in infected sickle cell anemia (SCA) patients worldwide. This study surveyed steady state SCA Nigerian patients for ASM to investigate the influence of malaria prevention behaviors and age on parasitaemia and multiplicity of infection (MOI). A total of 78 steady SCA patients aged 5 - 27 years on routine care at three health facilities in Lagos were investigated for ASM by light microscopy and PCR with a multiplicity of infection determined by genotyping block 2 of merozoite surface protein 1 (msp1) gene of Plasmodium falciparum (P. falciparum). Use of malaria prevention measures was captured using a semi-structured questionnaire. The prevalence rates of ASM (due to Pf only) by microscopy and PCR were found to be 27.3% and 47.4% respectively (P < 0.05) with a Mean + SEM parasite density of 2238.4 + 464.3 parasites/uL. Five distinct msp1 genotypes [K1 (2), MAD20 (2), RO33 (1)] were detected and significant (P
ISSN:0044-6025
1735-9694