Spirulina active substance mediated gut microbes improve lipid metabolism in high-fat diet fed rats

• Published in: Journal of functional foods 2019-08, Vol.59, p.215-222
• Main Authors: Chen, Hongpei, Zeng, Feng, Li, Shiming, Liu, Yilin, Gong, Shiyu, Lv, Xucong, Zhang, Jiachao, Liu, Bin
• Format: Article
• Language: English
• Subjects: Active substances; Gut microbes; High fat diet-fed rats; Lipid metabolism; Spirulina
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.04.049

[Display omitted] •SPMX was prepared by multi-level extraction and enzymatic hydrolysis.•SPMX showed hyperlipidemia effect in high-fat diet rat.•SPMX could improve the gut microbes of high-fat diet rat.•The relationship between serum lipid level and gut microbes was analyzed. This study aimed to investigate the effect of Spirulina mixed active substance (SPMX) on lipid metabolism and intestinal microbes in high-fat diet (HFD) rats. The effects of SPMX on body weight, serum lipids, pathological organization, gut microbiota and metabolites were analyzed. Results showed that body weight, serum lipids were significantly lower in SPMX group than that of HFD group, except HDL-C level. SPMX can enhance the restoration of fatty liver in HFD rats to a certain extent. SPMX obviously decreased the relative abundance of potential harmful bacteria. The mRNA expression levels of HMG-CoA, SREBP-1c and ACC in the liver tissue of the SPMX group were reduced remarkably compared with the HFD group. Spearman’s correlation analysis suggested that changes in feces microbiota was correlated with biochemical indicator. These findings suggested that SPMX could ameliorate lipid metabolism disorder, regulate intestinal microbial structure and can be used as lipid-lowering functional food.