Phylogeny and symbiotic effectiveness of indigenous rhizobial microsymbionts of common bean (Phaseolus vulgaris L.) in Malkerns, Eswatini

In most legumes, the rhizobial symbionts exhibit diversity across different environments. Although common bean ( Phaseolus vulgaris L.) is one of the important legumes in southern Africa, there is no available information on the genetic diversity and N 2 -fixing effectiveness of its symbionts in Mal...

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Published in:Scientific reports 2023-10, Vol.13 (1), p.17029-17029, Article 17029
Main Authors: Gunununu, Rotondwa P., Mohammed, Mustapha, Jaiswal, Sanjay K., Dakora, Felix D.
Format: Article
Language:English
Subjects:
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Beans
DnaK protein
Genetic diversity
Humanities and Social Sciences
Legumes
multidisciplinary
Multilocus sequence typing
NifH gene
Nitrogen fixation
Phaseolus vulgaris
Phylogeny
RpoB protein
rRNA 16S
Science
Science (multidisciplinary)
Sequence analysis
Stomata
Stomatal conductance
Symbionts
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Summary:In most legumes, the rhizobial symbionts exhibit diversity across different environments. Although common bean ( Phaseolus vulgaris L.) is one of the important legumes in southern Africa, there is no available information on the genetic diversity and N 2 -fixing effectiveness of its symbionts in Malkerns, Eswatini. In this study, we assessed the phylogenetic positions of rhizobial microsymbionts of common bean from Malkerns in Eswatini. The isolates obtained showed differences in morpho-physiology and N 2 -fixing efficiency. A dendrogram constructed from the ERIC-PCR banding patterns, grouped a total of 88 tested isolates into 80 ERIC-PCR types if considered at a 70% similarity cut-off point. Multilocus sequence analysis using 16S rRNA, rpoB, dnaK, gyrB, and glnII and symbiotic ( nifH and nodC ) gene sequences closely aligned the test isolates to the type strains of Rhizobium muluonense , R. paranaense , R. pusense, R. phaseoli and R. etli. Subjecting the isolates in this study to further description can potentially reveal novel species. Most of the isolates tested were efficient in fixing nitrogen and elicited greater stomatal conductance and photosynthetic rates in the common bean. Relative effectiveness (RE) varied from 18 to 433%, with 75 (85%) out of the 88 tested isolates being more effective than the nitrate fed control plants.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-43634-5