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WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis

WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) o...

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Published in:Nature communications 2019-04, Vol.10 (1), p.1498-15, Article 1498
Main Authors: Kaiser, Karol, Gyllborg, Daniel, Procházka, Jan, Salašová, Alena, Kompaníková, Petra, Molina, Francisco Lamus, Laguna-Goya, Rocio, Radaszkiewicz, Tomasz, Harnoš, Jakub, Procházková, Michaela, Potěšil, David, Barker, Roger A., Casado, Ángel Gato, Zdráhal, Zbyněk, Sedláček, Radislav, Arenas, Ernest, Villaescusa, J. Carlos, Bryja, Vítězslav
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Language:English
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Summary:WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) of the developing hindbrain, but not the telencephalon, in both mouse and human. Since the ChP produces and secretes the cerebrospinal fluid (CSF), we examine the presence of WNT5A in the CSF and find that it is associated with lipoprotein particles rather than exosomes. Moreover, since the CSF flows along the apical surface of hindbrain progenitors not expressing Wnt5a , we examined whether deletion of Wnt5a in the ChP controls their function and find that cerebellar morphogenesis is impaired. Our study thus identifies the CSF as a route and lipoprotein particles as a vehicle for long-range transport of biologically active WNT in the central nervous system. WNTs can signal over long distances but how this arises in the brain is unclear. Here, the authors show that WNT5A is secreted from the choroid plexus of the developing hindbrain (but not the telencephalon) and transported in the CSF with lipoprotein particles in order to control cerebellar morphogenesis.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-019-09298-4