The Response of Paracoccidioides lutzii to the Interaction with Human Neutrophils

The fungal pathogen Paracoccidioides lutzii causes systemic mycosis Paracoccidioidomycosis (PCM), which presents a broad distribution in Latin America. Upon infection, the fungus undergoes a morphological transition to yeast cells and provokes an inflammatory granulomatous reaction with a high numbe...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2023-11, Vol.9 (11), p.1088
Main Authors: Silva, Lana O’Hara Souza, Baeza, Lilian Cristiane, Pigosso, Laurine Lacerda, Silva, Kleber Santiago Freitas e, Pereira, Maristela, de Carvalho Júnior, Marcos Antonio Batista, de Almeida Soares, Célia Maria
Format: Article
Language:English
Subjects:
Chromatography
Detoxification
Fungi
genus Paracoccidioides
Glycolysis
Glyoxylate cycle
Heat shock
human neutrophils
Infections
Inflammation
Leukocytes (neutrophilic)
Leukocytes (polymorphonuclear)
Mass spectrometry
metabolism shunt
Microorganisms
Mycosis
Neutrophils
Paracoccidioides lutzii
Peptides
Proteins
Proteomics
Scientific imaging
South American blastomycosis
stress response
Yeast
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Summary:The fungal pathogen Paracoccidioides lutzii causes systemic mycosis Paracoccidioidomycosis (PCM), which presents a broad distribution in Latin America. Upon infection, the fungus undergoes a morphological transition to yeast cells and provokes an inflammatory granulomatous reaction with a high number of neutrophils in the lungs. In this work, we employed proteomic analysis to investigate the in vitro response of the fungus to the interaction with human neutrophils. Proteomic profiling of P. lutzii yeast cells harvested at 2 and 4 h post interaction with human polymorphonuclear cells allowed the identification of 505 proteins differentially accumulated. The data indicated that P. lutzii yeast cells underwent a shift in metabolism from glycolysis to Beta oxidation, increasing enzymes of the glyoxylate cycle and upregulating enzymes related to the detoxification of oxidative and heat shock stress. To our knowledge, this is the first study employing proteomic analysis in the investigation of the response of a member of the Paracoccidioides genus to the interaction with neutrophils.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof9111088