Methylprednisolone exerts neuroprotective effects by regulating autophagy and apoptosis

Methylprednisolone markedly reduces autophagy and apoptosis after secondary spinal cord injury. Here, we investigated whether pretreatment of cells with methylprednisolone would protect neuron-like cells from subsequent oxidative damage via suppression of autophagy and apoptosis. Cultured N2 a cells...

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Published in:Neural regeneration research 2016-05, Vol.11 (5), p.823-828
Main Authors: Gao, Wei, Chen, Shu-rui, Wu, Meng-yao, Gao, Kai, Li, Yuan-long, Wang, Hong-yu, Li, Chen-yuan, Li, Hong
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Autophagy
Autophagy (Cytology)
Cell growth
Flow cytometry
Methylprednisolone
Morphology
nerve regeneration
spinal cord injury
methylprednisolone
oxidative stress
N2a cells
autophagy
light chain 3B
Beclin-1
apoptosis
neuroprotection
H 2 O 2
neural regeneration
Oxidative stress
Polyclonal antibodies
Proteins
氧化损伤
甲基强的松龙
相差显微镜
神经保护作用
神经元样细胞
细胞凋亡
自噬
蛋白表达
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container_title Neural regeneration research
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creator Gao, Wei
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Li, Hong
description Methylprednisolone markedly reduces autophagy and apoptosis after secondary spinal cord injury. Here, we investigated whether pretreatment of cells with methylprednisolone would protect neuron-like cells from subsequent oxidative damage via suppression of autophagy and apoptosis. Cultured N2 a cells were pretreated with 10 μM methylprednisolone for 30 minutes, then exposed to 100 μM H2O2 for 24 hours. Inverted phase contrast microscope images, MTT assay, flow cytometry and western blot results showed that, compared to cells exposed to 100 μM H2O2 alone, cells pretreated with methylprednisolone had a significantly lower percentage of apoptotic cells, maintained a healthy morphology, and showed downregulation of autophagic protein light chain 3B and Beclin-1 protein expression. These findings indicate that methylprednisolone exerted neuroprotective effects against oxidative damage by suppressing autophagy and apoptosis.
doi_str_mv 10.4103/1673-5374.182711
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subjects Analysis
Apoptosis
Autophagy
Autophagy (Cytology)
Cell growth
Flow cytometry
Methylprednisolone
Morphology
nerve regeneration
spinal cord injury
methylprednisolone
oxidative stress
N2a cells
autophagy
light chain 3B
Beclin-1
apoptosis
neuroprotection
H 2 O 2
neural regeneration
Oxidative stress
Polyclonal antibodies
Proteins
氧化损伤
甲基强的松龙
相差显微镜
神经保护作用
神经元样细胞
细胞凋亡
自噬
蛋白表达
title Methylprednisolone exerts neuroprotective effects by regulating autophagy and apoptosis
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