Characterization of the Transcriptomes of Lgr5+ Hair Cell Progenitors and Lgr5- Supporting Cells in the Mouse Cochlea

Cochlear supporting cells (SCs) have been shown to be a promising resource for hair cell (HC) regeneration in the neonatal mouse cochlea. Previous studies have reported that Lgr5+ SCs can regenerate HCs both and and thus are considered to be inner ear progenitor cells. Lgr5+ progenitors are able to...

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Published in:Frontiers in molecular neuroscience 2017-04, Vol.10, p.122-122
Main Authors: Cheng, Cheng, Guo, Luo, Lu, Ling, Xu, Xiaochen, Zhang, ShaSha, Gao, Junyan, Waqas, Muhammad, Zhu, Chengwen, Chen, Yan, Zhang, Xiaoli, Xuan, Chuanying, Gao, Xia, Tang, Mingliang, Chen, Fangyi, Shi, Haibo, Li, Huawei, Chai, Renjie
Format: Article
Language:English
Subjects:
Cell cycle
Cell growth
Cochlea
differentiation
Flow cytometry
Gene expression
Hair
Hearing loss
Hospitals
Inner ear
Laboratory animals
Neonates
Neuroscience
Neurosciences
Otolaryngology
Progenitor cells
proliferation
Protein interaction
Proteins
regeneration
Ribonucleic acid
RNA
RNA-Seq
Signal transduction
sphere
Stem cells
Surgery
Therapeutic applications
Transcription factors
Transgenic animals
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Summary:Cochlear supporting cells (SCs) have been shown to be a promising resource for hair cell (HC) regeneration in the neonatal mouse cochlea. Previous studies have reported that Lgr5+ SCs can regenerate HCs both and and thus are considered to be inner ear progenitor cells. Lgr5+ progenitors are able to regenerate more HCs than Lgr5- SCs, and it is important to understand the mechanism behind the proliferation and HC regeneration of these progenitors. Here, we isolated Lgr5+ progenitors and Lgr5- SCs from Lgr5-EGFP-CreERT2/Sox2-CreERT2/Rosa26-tdTomato mice via flow cytometry. As expected, we found that Lgr5+ progenitors had significantly higher proliferation and HC regeneration ability than Lgr5- SCs. Next, we performed RNA-Seq to determine the gene expression profiles of Lgr5+ progenitors and Lgr5- SCs. We analyzed the genes that were enriched and differentially expressed in Lgr5+ progenitors and Lgr5- SCs, and we found 8 cell cycle genes, 9 transcription factors, and 24 cell signaling pathway genes that were uniquely expressed in one population but not the other. Last, we made a protein-protein interaction network to further analyze the role of these differentially expressed genes. In conclusion, we present a set of genes that might regulate the proliferation and HC regeneration ability of Lgr5+ progenitors, and these might serve as potential new therapeutic targets for HC regeneration.
ISSN:1662-5099
1662-5099
DOI:10.3389/fnmol.2017.00122