COMPARISON OF FIVE METHODS FOR OXACILLIN SUSCEPTIBILITY TESTING OF Staphylococcus aureus ISOLATES FROM CYSTIC FIBROSIS PATIENTS

Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy o...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 2012-12, Vol.54 (6), p.305-306
Main Authors: MIMICA, Marcelo J, CARVALHO, Rozane L, BEREZIN, Eitan N, DAMACENO, Neiva, CAIAFFA-FILHO, Hélio H
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bacterial diseases
Bacterial Proteins - genetics
Biological and medical sciences
Cefoxitin
Culture Media - chemistry
Cystic fibrosis
Cystic Fibrosis - microbiology
Errors of metabolism
General aspects
Human bacterial diseases
Humans
Infectious diseases
mecA
Medical sciences
Metabolic diseases
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Microbial Sensitivity Tests - methods
Miscellaneous hereditary metabolic disorders
MRSA
Oxacillin
Oxacillin - pharmacology
Penicillin-Binding Proteins
Phenotype
Reproducibility of Results
Sensitivity and Specificity
Staphylococcal Infections - microbiology
Staphylococcal infections, streptococcal infections, pneumococcal infections
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - drug effects
TROPICAL MEDICINE
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Summary:Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.
ISSN:0036-4665
1678-9946
1678-9946
0036-4665
DOI:10.1590/S0036-46652012000600002