Evaluating the effectiveness of quantitative pupillometry in assessing dynamic aerobic training intensity

• Published in: Scientific reports 2024-10, Vol.14 (1), p.26071-9, Article 26071
• Main Authors: Dai, Junlong, Wang, Xingsheng, Hang, Chenchen, An, Le, Shao, Rui, Tang, Ziren
• Format: Article
• Language: English
• Subjects: 631/114/2413; 692/1807/1486; 692/308/409; Adult; Athletes; Dynamic aerobic training; Exercise - physiology; Exercise load; Exercise Test - methods; Heart Rate - physiology; Humanities and Social Sciences; Humans; Male; multidisciplinary; Physical Exertion - physiology; Pupil - physiology; Pupillary light reflex; Quantitative pupillometry; Ratings of perceived exertion; Reflex, Pupillary - physiology; ROC Curve; Science; Science (multidisciplinary); Young Adult
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-77588-z

Sports injuries often arise from improper scheduling of exercise loads, and timely assessment of these loads is essential for minimizing injury risk. This article investigates the validity of using changes in pupillary light reflex (PLR) during dynamic aerobic training as a novel approach to evaluating exercise load. Dynamic aerobic training was conducted on a power bicycle for 15 min. With a 3-minute interval as the demarcation line, PLR measurement was performed before training and heart rate was recorded throughout the process. The Rating of Perceived Exertion (RPE) scale invented by Borg was used for scoring before training and after training. The normal distribution of data was confirmed through the Shapiro-Wilk test. Pearson correlation analysis was used to quantify the correlation between variables. The Receiver Operating Characteristic (ROC) curve and the area under the curve (AUC) analysis were used to determine the indicators of exercise load. The optimal threshold of the indicators was calculated through the Youden index to evaluate sensitivity and specificity. Thirty male second-tier athletes with a mean age of 23.66 ± 2.21 years, a mean height of 175.3 ± 6.5 cm, and a mean weight of 68.99 ± 10.35 kg participated in this study. Based on the RPE scale results, it was confirmed that the 15-minute dynamic aerobic exercise successfully elicited varying levels of perceived exertion among the athletes. The findings of this study indicate significant changes in PLR and heart rate (HR) with increasing exercise duration and external load. There were strong correlations between RPE and maximum constriction velocity (MCV) (|r| = 0.8309, p