Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes

• Published in: Neuropsychiatric disease and treatment 2019-04, Vol.15, p.927-934
• Main Authors: Wang, Yirong, Fang, Kewei, He, Shijia, Fan, Yang, Yu, Juming, Zhang, Xiaodong
• Format: Article
• Language: English
• Subjects: Brain; Catalase; EDTA; Enzyme-linked immunosorbent assay; Enzymes; Hippocampus; Iron; Iron compounds; iron-containing enzymes; Kinases; Magnetic fields; neuromechanisms; Neurons; Nonferrous metals; Original Research; Protein kinase A; Protein kinases; repetitive magnetic stimulation; Time; Transcranial magnetic stimulation; Western blotting
• ISSN: 1176-6328; 1178-2021; 1178-2021
• DOI: 10.2147/NDT.S199328

The mechanism of action of repetitive transcranial magnetic stimulation (rTMS) involves the generation of neuronal and action potentials utilizing induced currents in time-varying magnetic fields. However, the long-lasting and effective biological impact of magnetic stimulation does not appear to be completely explained by the transient magnetic field pulses. In this context, we hypothesized magnetic stimulation may affect the expression of iron-containing enzymes in neurons, mediating the long-lasting biological effects associated with this stimulus. Primarily cultured hippocampus neurons from SD rats were used as the cell model in this study. These were randomly divided into control, sham, and magnetic stimulation groups to probe into the effect of the magnetic field directly. The latter group received 40%, 60%, and 100% maximal stimulator output Tesla (1.68, 2.52, and 4.2 T) with low-frequency rTMS (1 Hz). The expression of iron-containing enzymes (catalase and aconitase) and non-ferrous enzymes (protein kinase A) was measured with Western blotting and ELISA. The survival rates of neurons in the 40%T and 60%T groups were significantly increased in comparison to the controls (