Characterization of a Bacteriophage GEC_vB_Bfr_UZM3 Active against Bacteroides fragilis

is a commensal gut bacterium that is associated with a number of blood and tissue infections. It has not yet been recognized as one of the drug-resistant human pathogens, but cases of the refractory infections, caused by strains that are not susceptible to the common antibiotic regimes established f...

Full description

Saved in:
Bibliographic Details
Published in:Viruses 2023-04, Vol.15 (5), p.1042
Main Authors: Bakuradze, Nata, Merabishvili, Maia, Kusradze, Ia, Ceyssens, Pieter-Jan, Onsea, Jolien, Metsemakers, Willem-Jan, Grdzelishvili, Nino, Natroshvili, Guliko, Tatrishvili, Tamar, Lazvliashvili, Davit, Mitskevich, Nunu, Pirnay, Jean-Paul, Chanishvili, Nina
Format: Article
Language:English
Subjects:
Abscesses
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antibiotics
Bacteria
Bacterial Infections
bacteriophage
Bacteriophages - genetics
Bacteroides fragilis
Body temperature
Chronic infection
Diarrhea
Drug resistance
Genomes
Humans
Infections
Mixed infection
Multidrug resistance
Osteomyelitis
phage therapy
Phages
Virulence
Whole genome sequencing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:is a commensal gut bacterium that is associated with a number of blood and tissue infections. It has not yet been recognized as one of the drug-resistant human pathogens, but cases of the refractory infections, caused by strains that are not susceptible to the common antibiotic regimes established for have been more frequently reported. Bacteriophages (phages) were found to be a successful antibacterial alternative to antibiotic therapy in many cases of multidrug-resistant (MDR) bacterial infections. We have characterized the bacteriophage GEC_vB_Bfr_UZM3 (UZM3), which was used for the treatment of a patient with a chronic osteomyelitis caused by a mixed infection. Studied biological and morphological properties of UZM3 showed that it seems to represent a strictly lytic phage belonging to a siphovirus morphotype. It is characterized by high stability at body temperature and in pH environments for about 6 h. Whole genome sequencing analysis of the phage UZM3 showed that it does not harbor any known virulence genes and can be considered as a potential therapeutic phage to be used against infections.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15051042