Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)

Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of c...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-04, Vol.13 (4), p.455
Main Authors: Tannenberg, Robert, Paul, Martin, Röder, Bettina, Gande, Santosh L, Sreeramulu, Sridhar, Saxena, Krishna, Richter, Christian, Schwalbe, Harald, Swart, Claudia, Weller, Michael G
Format: Article
Language:English
Subjects:
acute myocardial infarction (AMI)
Analysis
Antibodies
biomarker
Biomarkers
Biosensing Techniques
Biosensors
Blood plasma
cardiac troponin I (cTnI)
Cardiovascular diseases
Care and treatment
Chemiluminescence
Coronary artery disease
Cross-reactivity
Diagnosis
emergency
Health aspects
heart attack
Heart diseases
Humans
Immunoassay
Immunosensors
Laboratories
Luminescence
Microfluidics
Myocardial infarction
Myocardial Infarction - diagnosis
Peptides
Proteins
Risk factors
Troponin I
Vascular diseases
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Summary:Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of cTnI with the potential of online measurements, a chemiluminescence-based immunosensor is presented as a proof of concept. A flow cell was designed and combined with a sensitive CMOS camera allowing sensitive optical readout. In addition, a microfluidic setup was established, which achieved selective and quasi-online cTnI determination within ten minutes. The sensor was tested with recombinant cTnI in phosphate buffer and demonstrated cTnI measurements in the concentration range of 2-25 µg/L. With the optimized system, a limit of detection (LoD) of 0.6 µg/L (23 pmol/L) was achieved. Furthermore, the selectivity of the immunosensor was investigated with other recombinant proteins, such as cTnT, and cTnC, at a level of 16 µg/L. No cross-reactivity could be observed. Measurements with diluted blood plasma and serum resulted in an LoD of 60 µg/L (2.4 nmol/L) and 70 µg/L (2.9 nmol/L), respectively.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13040455