Beneficial effects of green coffee and green tea extract combination on metabolic syndrome improvement by affecting AMPK And PPAR-α gene expression

• Published in: Journal of advanced pharmaceutical technology and research 2020-04, Vol.11 (2), p.81-85
• Main Authors: Lukitasari, Mifetika, Nugroho, Dwi, Rohman, Mohammad, Widodo, Nashi, Farmawati, Arta, Hastuti, Pramudji
• Format: Article
• Language: English
• Subjects: Adenosine kinase; adenosine monophosphate-activated protein kinase-α2; AMP; Blood pressure; Cholesterol; Chromatography; Coffee; Fatty acids; Gene expression; Glucose; green coffee; Green tea; Homeostasis; Hyperglycemia; Kinases; Liver; Metabolic syndrome; Original; peroxisome proliferator-activated receptor α; Peroxisome proliferator-activated receptors; Polymerase chain reaction; Protein kinase; Tea
• ISSN: 2231-4040; 0976-2094
• DOI: 10.4103/japtr.JAPTR_116_19

Effect of green coffee and green tea extract on metabolic syndrome. To explore green coffee and green tea extract combination effect on metabolic profile and blood pressure improvement through adenosine monophosphate-activated protein kinase (AMPK) and Peroxisome Proliferator-Activated Receptor α (PPARα) gene expression modulation. Experimental laboratory research with pre- and post-control group design. Twenty-five metabolic syndrome rats model were grouped into five groups (n = 5): standard control (normal), metabolic syndrome (SM), green coffee extract (GC), green tea extract (GT), and combination green coffee and green tea extract (CM). The extract was given during 9 weeks. Serum glucose, triglyceride, high-density lipoprotein, and systolic blood pressure level were analyzed before and after the extract administration. At the end of the study, PPAR-α and AMPK-α2 gene were analyzed. Independent t-test. CM group had significantly higher PPAR-α, and AMPK-α2 gene expression compared to those of SM, GC, and GT group. Green coffee and green tea extract combination administration improved metabolic profile and blood pressure on metabolic syndrome through affecting PPAR-α and AMPK-α2 gene expression.