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Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills
Juniperus communis L. represents a multi-purpose crop used in the pharmaceutical, food, and cosmetic industry. Several studies present the possible medicinal properties of different Juniperus taxa native to specific geographical area. The present study aims to evaluate the genoprotective, antioxidan...
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| Published in: | BMC complementary and alternative medicine 2018-01, Vol.18 (1), p.3-3, Article 3 |
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| creator | Fierascu, Irina Ungureanu, Camelia Avramescu, Sorin Marius Cimpeanu, Carmen Georgescu, Mihaela Ioana Fierascu, Radu Claudiu Ortan, Alina Sutan, Anca Nicoleta Anuta, Valentina Zanfirescu, Anca Dinu-Pirvu, Cristina Elena Velescu, Bruno Stefan |
| description | Juniperus communis L. represents a multi-purpose crop used in the pharmaceutical, food, and cosmetic industry. Several studies present the possible medicinal properties of different Juniperus taxa native to specific geographical area. The present study aims to evaluate the genoprotective, antioxidant, antifungal and anti-inflammatory potential of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills.
The prepared hydroethanolic extract of Juniperus communis L. was characterized by GC-MS, HPLC, UV-Vis spectrometry and phytochemical assays. The antioxidant potential was evaluated using the DPPH assay, the antifungal effect was studied on Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323, while the genoprotective effect was evaluated using the Allium cepa assay. The anti-inflammatory effect was evaluated in two inflammation experimental models (dextran and kaolin) by plethysmometry. Male Wistar rats were treated by gavage with distilled water (negative control), the microemulsion (positive control), diclofenac sodium aqueous solution (reference) and microemulsions containing juniper extract (experimental group). The initial paw volume and the paw volumes at 1, 2, 3, 4, 5 and 24 h were measured.
Total terpenoids, phenolics and flavonoids were estimated to be 13.44 ± 0.14 mg linalool equivalent, 19.23 ± 1.32 mg gallic acid equivalent, and 5109.6 ± 21.47 mg rutin equivalent per 100 g of extract, respectively. GC-MS characterization of the juniper extract identified 57 volatile compounds in the sample, while the HPLC analysis revealed the presence of the selected compounds (α-pinene, chlorogenic acid, rutin, apigenin, quercitin). The antioxidant potential of the crude extract was found to be 81.63 ± 0.38% (measured by the DPPH method). The results of the antifungal activity assay (for Aspergillus niger and Penicillium hirsutum) were 21.6 mm, respectively 17.2 mm as inhibition zone. Test results demonstrated the genoprotective potential of J. communis undiluted extract, inhibiting the mitodepressive effect of ethanol. The anti-inflammatory action of the juniper extract, administered as microemulsion in acute-dextran model was increased when compared to kaolin subacute inflammation induced model.
The hydroalcoholic extract obtained from wild-growing Juniperus communis native to Romanian southern sub-Carpathian hills has genoprotective, antioxidant, antifungal and anti-inflammatory |
| doi_str_mv | 10.1186/s12906-017-2066-8 |
| format | article |
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The prepared hydroethanolic extract of Juniperus communis L. was characterized by GC-MS, HPLC, UV-Vis spectrometry and phytochemical assays. The antioxidant potential was evaluated using the DPPH assay, the antifungal effect was studied on Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323, while the genoprotective effect was evaluated using the Allium cepa assay. The anti-inflammatory effect was evaluated in two inflammation experimental models (dextran and kaolin) by plethysmometry. Male Wistar rats were treated by gavage with distilled water (negative control), the microemulsion (positive control), diclofenac sodium aqueous solution (reference) and microemulsions containing juniper extract (experimental group). The initial paw volume and the paw volumes at 1, 2, 3, 4, 5 and 24 h were measured.
Total terpenoids, phenolics and flavonoids were estimated to be 13.44 ± 0.14 mg linalool equivalent, 19.23 ± 1.32 mg gallic acid equivalent, and 5109.6 ± 21.47 mg rutin equivalent per 100 g of extract, respectively. GC-MS characterization of the juniper extract identified 57 volatile compounds in the sample, while the HPLC analysis revealed the presence of the selected compounds (α-pinene, chlorogenic acid, rutin, apigenin, quercitin). The antioxidant potential of the crude extract was found to be 81.63 ± 0.38% (measured by the DPPH method). The results of the antifungal activity assay (for Aspergillus niger and Penicillium hirsutum) were 21.6 mm, respectively 17.2 mm as inhibition zone. Test results demonstrated the genoprotective potential of J. communis undiluted extract, inhibiting the mitodepressive effect of ethanol. The anti-inflammatory action of the juniper extract, administered as microemulsion in acute-dextran model was increased when compared to kaolin subacute inflammation induced model.
The hydroalcoholic extract obtained from wild-growing Juniperus communis native to Romanian southern sub-Carpathian hills has genoprotective, antioxidant, antifungal and anti-inflammatory properties.</description><identifier>ISSN: 1472-6882</identifier><identifier>EISSN: 1472-6882</identifier><identifier>DOI: 10.1186/s12906-017-2066-8</identifier><identifier>PMID: 29301523</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Analgesics ; Animals ; Anti-inflammatory agents ; Anti-Inflammatory Agents - chemistry ; Anti-Inflammatory Agents - pharmacology ; Antifungal activity ; Antifungal agents ; Antifungal Agents - chemistry ; Antifungal Agents - pharmacology ; Antioxidants ; Aqueous solutions ; Aspergillus niger ; Aspergillus niger - drug effects ; Assaying ; Biphenyl Compounds - analysis ; Biphenyl Compounds - metabolism ; Chlorogenic acid ; Cupressaceae ; Dextran ; Diclofenac ; Distilled water ; Diuretics ; Edema ; Equivalence ; Ethanol ; Flavonoids ; Flavonoids - chemistry ; Flavonoids - pharmacology ; Folk medicine ; Food industry ; Fungicides ; Gallic acid ; Genoprotective ; High-performance liquid chromatography ; Hills ; Hydroalcoholic extract ; Inflammation ; Inflammation - metabolism ; Juniperus - chemistry ; Juniperus communis ; Kaolin ; Linalool ; Liquid chromatography ; Male ; Microemulsions ; Natural compounds ; Natural products ; Oils & fats ; Penicillium ; Penicillium - drug effects ; Phenols ; Phenols - chemistry ; Phenols - pharmacology ; Phytochemicals ; Picrates - analysis ; Picrates - metabolism ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Protective Agents - chemistry ; Protective Agents - pharmacology ; Rats ; Rats, Wistar ; Romania ; Rutin ; Sodium ; Spectrometry ; Terpenes ; Volatile compounds ; α-Pinene</subject><ispartof>BMC complementary and alternative medicine, 2018-01, Vol.18 (1), p.3-3, Article 3</ispartof><rights>Copyright © 2018. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s). 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4088-d16924329854eb067f43c2a2afe03610d5aa43c84d5c5b6968262c0f070cddf23</citedby><cites>FETCH-LOGICAL-c4088-d16924329854eb067f43c2a2afe03610d5aa43c84d5c5b6968262c0f070cddf23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755145/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755145/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29301523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fierascu, Irina</creatorcontrib><creatorcontrib>Ungureanu, Camelia</creatorcontrib><creatorcontrib>Avramescu, Sorin Marius</creatorcontrib><creatorcontrib>Cimpeanu, Carmen</creatorcontrib><creatorcontrib>Georgescu, Mihaela Ioana</creatorcontrib><creatorcontrib>Fierascu, Radu Claudiu</creatorcontrib><creatorcontrib>Ortan, Alina</creatorcontrib><creatorcontrib>Sutan, Anca Nicoleta</creatorcontrib><creatorcontrib>Anuta, Valentina</creatorcontrib><creatorcontrib>Zanfirescu, Anca</creatorcontrib><creatorcontrib>Dinu-Pirvu, Cristina Elena</creatorcontrib><creatorcontrib>Velescu, Bruno Stefan</creatorcontrib><title>Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills</title><title>BMC complementary and alternative medicine</title><addtitle>BMC Complement Altern Med</addtitle><description>Juniperus communis L. represents a multi-purpose crop used in the pharmaceutical, food, and cosmetic industry. Several studies present the possible medicinal properties of different Juniperus taxa native to specific geographical area. The present study aims to evaluate the genoprotective, antioxidant, antifungal and anti-inflammatory potential of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills.
The prepared hydroethanolic extract of Juniperus communis L. was characterized by GC-MS, HPLC, UV-Vis spectrometry and phytochemical assays. The antioxidant potential was evaluated using the DPPH assay, the antifungal effect was studied on Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323, while the genoprotective effect was evaluated using the Allium cepa assay. The anti-inflammatory effect was evaluated in two inflammation experimental models (dextran and kaolin) by plethysmometry. Male Wistar rats were treated by gavage with distilled water (negative control), the microemulsion (positive control), diclofenac sodium aqueous solution (reference) and microemulsions containing juniper extract (experimental group). The initial paw volume and the paw volumes at 1, 2, 3, 4, 5 and 24 h were measured.
Total terpenoids, phenolics and flavonoids were estimated to be 13.44 ± 0.14 mg linalool equivalent, 19.23 ± 1.32 mg gallic acid equivalent, and 5109.6 ± 21.47 mg rutin equivalent per 100 g of extract, respectively. GC-MS characterization of the juniper extract identified 57 volatile compounds in the sample, while the HPLC analysis revealed the presence of the selected compounds (α-pinene, chlorogenic acid, rutin, apigenin, quercitin). The antioxidant potential of the crude extract was found to be 81.63 ± 0.38% (measured by the DPPH method). The results of the antifungal activity assay (for Aspergillus niger and Penicillium hirsutum) were 21.6 mm, respectively 17.2 mm as inhibition zone. Test results demonstrated the genoprotective potential of J. communis undiluted extract, inhibiting the mitodepressive effect of ethanol. The anti-inflammatory action of the juniper extract, administered as microemulsion in acute-dextran model was increased when compared to kaolin subacute inflammation induced model.
The hydroalcoholic extract obtained from wild-growing Juniperus communis native to Romanian southern sub-Carpathian hills has genoprotective, antioxidant, antifungal and anti-inflammatory properties.</description><subject>Analgesics</subject><subject>Animals</subject><subject>Anti-inflammatory agents</subject><subject>Anti-Inflammatory Agents - chemistry</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antifungal activity</subject><subject>Antifungal agents</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antioxidants</subject><subject>Aqueous solutions</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - drug effects</subject><subject>Assaying</subject><subject>Biphenyl Compounds - analysis</subject><subject>Biphenyl Compounds - metabolism</subject><subject>Chlorogenic acid</subject><subject>Cupressaceae</subject><subject>Dextran</subject><subject>Diclofenac</subject><subject>Distilled water</subject><subject>Diuretics</subject><subject>Edema</subject><subject>Equivalence</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Flavonoids - chemistry</subject><subject>Flavonoids - pharmacology</subject><subject>Folk medicine</subject><subject>Food industry</subject><subject>Fungicides</subject><subject>Gallic acid</subject><subject>Genoprotective</subject><subject>High-performance liquid chromatography</subject><subject>Hills</subject><subject>Hydroalcoholic extract</subject><subject>Inflammation</subject><subject>Inflammation - metabolism</subject><subject>Juniperus - chemistry</subject><subject>Juniperus communis</subject><subject>Kaolin</subject><subject>Linalool</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Microemulsions</subject><subject>Natural compounds</subject><subject>Natural products</subject><subject>Oils & fats</subject><subject>Penicillium</subject><subject>Penicillium - drug effects</subject><subject>Phenols</subject><subject>Phenols - chemistry</subject><subject>Phenols - pharmacology</subject><subject>Phytochemicals</subject><subject>Picrates - analysis</subject><subject>Picrates - metabolism</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Protective Agents - chemistry</subject><subject>Protective Agents - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Romania</subject><subject>Rutin</subject><subject>Sodium</subject><subject>Spectrometry</subject><subject>Terpenes</subject><subject>Volatile compounds</subject><subject>α-Pinene</subject><issn>1472-6882</issn><issn>1472-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdUsFu1DAQjRCIlsIHcEGWuBSJFNuJE_uChFZQilZCQnC2Jraz8cqxFzvZdv-Qz8LplqrlNDNv3jzNjF5RvCb4ghDefEiECtyUmLQlxU1T8ifFKalbWjac06cP8pPiRUpbnImc1M-LEyoqTBitTos_l8aHXQyTUZPdm_cI_GTDjdU5Hot-9htwOdW3ZWl972AcYQrxgMwe3Ax5wqPQo-GgYwCnwhCcVcjcTBHUtHSurdPlJoZr6zfo2-ztzsQ5IRXGMRcJrS_Q-WreRZMSKAPmHfKw7IOmgH6EEbwFj1KYp8HEnMxduYK4g2lY8ME6l14Wz3pwyby6i2fFry-ff66-luvvl1erT-tS1ZjzUpNG0LqigrPadLhp-7pSFCj0BlcNwZoBZITXminWNaLhtKEK97jFSuueVmfF1VFXB9jKXbQjxIMMYOUtEOJGQpysckZ2TauVxsRooepeEC4Ia2ujeoJpR1mbtT4etXZzNxqtjM8Pc49EH3e8HeQm7CVrGSM1ywLndwIx_J5NmuRokzLOgTdhTpIILljFWlFn6tv_qNswR59fJSmp2lq0gvLMIkeWiiGlaPr7ZQiWi-fk0XMyW0kunpPLzJuHV9xP_DNZ9Rd9ZdeM</recordid><startdate>20180104</startdate><enddate>20180104</enddate><creator>Fierascu, Irina</creator><creator>Ungureanu, Camelia</creator><creator>Avramescu, Sorin Marius</creator><creator>Cimpeanu, Carmen</creator><creator>Georgescu, Mihaela Ioana</creator><creator>Fierascu, Radu Claudiu</creator><creator>Ortan, Alina</creator><creator>Sutan, Anca Nicoleta</creator><creator>Anuta, Valentina</creator><creator>Zanfirescu, Anca</creator><creator>Dinu-Pirvu, Cristina Elena</creator><creator>Velescu, Bruno Stefan</creator><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20180104</creationdate><title>Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>BMC complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fierascu, Irina</au><au>Ungureanu, Camelia</au><au>Avramescu, Sorin Marius</au><au>Cimpeanu, Carmen</au><au>Georgescu, Mihaela Ioana</au><au>Fierascu, Radu Claudiu</au><au>Ortan, Alina</au><au>Sutan, Anca Nicoleta</au><au>Anuta, Valentina</au><au>Zanfirescu, Anca</au><au>Dinu-Pirvu, Cristina Elena</au><au>Velescu, Bruno Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills</atitle><jtitle>BMC complementary and alternative medicine</jtitle><addtitle>BMC Complement Altern Med</addtitle><date>2018-01-04</date><risdate>2018</risdate><volume>18</volume><issue>1</issue><spage>3</spage><epage>3</epage><pages>3-3</pages><artnum>3</artnum><issn>1472-6882</issn><eissn>1472-6882</eissn><abstract>Juniperus communis L. represents a multi-purpose crop used in the pharmaceutical, food, and cosmetic industry. Several studies present the possible medicinal properties of different Juniperus taxa native to specific geographical area. The present study aims to evaluate the genoprotective, antioxidant, antifungal and anti-inflammatory potential of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills.
The prepared hydroethanolic extract of Juniperus communis L. was characterized by GC-MS, HPLC, UV-Vis spectrometry and phytochemical assays. The antioxidant potential was evaluated using the DPPH assay, the antifungal effect was studied on Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323, while the genoprotective effect was evaluated using the Allium cepa assay. The anti-inflammatory effect was evaluated in two inflammation experimental models (dextran and kaolin) by plethysmometry. Male Wistar rats were treated by gavage with distilled water (negative control), the microemulsion (positive control), diclofenac sodium aqueous solution (reference) and microemulsions containing juniper extract (experimental group). The initial paw volume and the paw volumes at 1, 2, 3, 4, 5 and 24 h were measured.
Total terpenoids, phenolics and flavonoids were estimated to be 13.44 ± 0.14 mg linalool equivalent, 19.23 ± 1.32 mg gallic acid equivalent, and 5109.6 ± 21.47 mg rutin equivalent per 100 g of extract, respectively. GC-MS characterization of the juniper extract identified 57 volatile compounds in the sample, while the HPLC analysis revealed the presence of the selected compounds (α-pinene, chlorogenic acid, rutin, apigenin, quercitin). The antioxidant potential of the crude extract was found to be 81.63 ± 0.38% (measured by the DPPH method). The results of the antifungal activity assay (for Aspergillus niger and Penicillium hirsutum) were 21.6 mm, respectively 17.2 mm as inhibition zone. Test results demonstrated the genoprotective potential of J. communis undiluted extract, inhibiting the mitodepressive effect of ethanol. The anti-inflammatory action of the juniper extract, administered as microemulsion in acute-dextran model was increased when compared to kaolin subacute inflammation induced model.
The hydroalcoholic extract obtained from wild-growing Juniperus communis native to Romanian southern sub-Carpathian hills has genoprotective, antioxidant, antifungal and anti-inflammatory properties.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>29301523</pmid><doi>10.1186/s12906-017-2066-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1472-6882 |
| ispartof | BMC complementary and alternative medicine, 2018-01, Vol.18 (1), p.3-3, Article 3 |
| issn | 1472-6882 1472-6882 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_b67dcd01ed9c4f91891574ecf102b257 |
| source | PubMed Central |
| subjects | Analgesics Animals Anti-inflammatory agents Anti-Inflammatory Agents - chemistry Anti-Inflammatory Agents - pharmacology Antifungal activity Antifungal agents Antifungal Agents - chemistry Antifungal Agents - pharmacology Antioxidants Aqueous solutions Aspergillus niger Aspergillus niger - drug effects Assaying Biphenyl Compounds - analysis Biphenyl Compounds - metabolism Chlorogenic acid Cupressaceae Dextran Diclofenac Distilled water Diuretics Edema Equivalence Ethanol Flavonoids Flavonoids - chemistry Flavonoids - pharmacology Folk medicine Food industry Fungicides Gallic acid Genoprotective High-performance liquid chromatography Hills Hydroalcoholic extract Inflammation Inflammation - metabolism Juniperus - chemistry Juniperus communis Kaolin Linalool Liquid chromatography Male Microemulsions Natural compounds Natural products Oils & fats Penicillium Penicillium - drug effects Phenols Phenols - chemistry Phenols - pharmacology Phytochemicals Picrates - analysis Picrates - metabolism Plant Extracts - chemistry Plant Extracts - pharmacology Protective Agents - chemistry Protective Agents - pharmacology Rats Rats, Wistar Romania Rutin Sodium Spectrometry Terpenes Volatile compounds α-Pinene |
| title | Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills |
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