Prevalence of asymptomatic P. falciparum gametocyte carriage among school children in Mbita, Western Kenya and assessment of the association between gametocyte density, multiplicity of infection and mosquito infection prevalence

• Published in: Wellcome open research 2020, Vol.5, p.259
• Main Authors: Touray, Abdoulie O, Mobegi, Victor A, Wamunyokoli, Fred, Butungi, Hellen, Herren, Jeremy K
• Format: Article
• Language: English
• ISSN: 2398-502X; 2398-502X
• DOI: 10.12688/wellcomeopenres.16299.2

Asymptomatic gametocyte carriers are reservoirs for sustaining transmission in malaria endemic regions. Gametocyte presence in the host peripheral blood is a predictor of capacity to transmit malaria. However, it does not always directly translate to mosquito infectivity. Factors that affect mosquito infectivity include, gametocyte sex-ratio and density, multiplicity of infection (MOI), and host and vector anti-parasite immunity. We assess the prevalence of gametocyte carriage and some of its associated risk factors among asymptomatic schoolchildren in Western Kenya and to further analyse the association between gametocyte density, multiplicity of infection (MOI) and mosquito infection prevalence. parasite infections were detected by RDT (Rapid Diagnostic Test) and microscopy among schoolchildren (5-15 years old). Blood from 37 microscopy positive gametocyte carriers offered to laboratory reared mosquitoes. A total of 3395 fully fed mosquitoes were screened for sporozoites by ELISA. was genotyped using 10 polymorphic microsatellite markers. The association between MOI and gametocyte density and mosquito infection prevalence was investigated. A significantly higher prevalence of infection was found in males 31.54% (764/2422) ( -value < 0.001) compared to females 26.72% (657/2459). The microscopic gametocyte prevalence among the study population was 2% (84/4881). Children aged 5-9 years have a higher prevalence of gametocyte carriage (odds ratios = 2.1 [95% CI = 1.3-3.4], = 0.002) as compared to children aged 10-15 years. After offering gametocyte positive blood to by membrane feeding assay, our results indicated that 68.1% of the variation in mosquito infection prevalence was accounted for by gametocyte density and MOI (R-SQR. = 0.681,