Oligandrin from Croton oligandrus (Euphorbiaceae) exhibits anti-breast cancer activity through immune-boosting mechanisms: In vitro and in vivo study

• Published in: Heliyon 2024-08, Vol.10 (15), p.e35000, Article e35000
• Main Authors: Zingue, Stéphane, Fotsing Fongang, Yannick Stéphane, Ossomba, Eric Roger, Tatsinda, Vanneck, Silihe, Kevine Kamga, Mbou, William Defo, Fogang, Balotin, Essomba, René, Chouna, Jean Rodolphe, Njamen, Dieudonné, Ayong, Lawrence
• Format: Article
• Language: English
• Subjects: Breast cancer; Croton oligandrus; Cytokines; Immune system; Oligandrin; Oxidative stress
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e35000

Recent developments in cancer research indicate that cancer is a manifestation of immune system dysfunction. Many natural anticancer agents developed recently possess immune-modulatory properties. In our ongoing pursuit of anticancer alternatives, we evaluated the immune-modulatory potential of oligandrin, an ent-pimarane type diterpenoid from Croton oligatrus. we assessed on Breast cancer patients’ peripheral blood mononuclear cells (PBMCs) were isolated to assess the effect of oligandrin (0.5, 1, 10, 100, 200 mg/mL) in vitro using the Ficoll-histopaque density centrifugation method. The parameters that were assessed included, PBMC viability and cytokine (IL-6, IL-12, IL-10, EGF, TNF-α, INF-γ) production. In vivo, we chemically induced breast cancer using DMBA (50 mg/kg BW) in Wistar rats, then treated them with oligandrin (1 mg/kg BW) or standards (tamoxifen 3.3 mg/kg; letrozole 1 mg/kg) for 20 weeks. The parameters that were evaluated included, tumor burden, volume, incidence, histopathology, antioxidant, and inflammatory status. Oligandrin (1, 10, 100 and 200 μg/mL) significantly increased (p