Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography-Photodiode Array Detector and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Bopyeo-tang (BPT) is composed of six medicinal herbs ( L., (Gaertn.) DC., C.A.Mey., L.f., Schischkin, and (Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydro...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2024-03, Vol.29 (5), p.1171
Main Author: Seo, Chang-Seob
Format: Article
Language:English
Subjects:
Acetonitrile
Acids
Bopyeo-tang
Chromatography
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid
Detectors
Drugs, Chinese Herbal - chemistry
Herbal medicine
High performance liquid chromatography
HPLC–PDA
Mass spectrometry
Medical research
Medicine
Medicine, Experimental
Pulmonary fibrosis
Quantitative analysis
Republic of Korea
Scientific imaging
simultaneous quantification
Tandem Mass Spectrometry - methods
traditional Korean medicine
UPLC–MS/MS
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Summary:Bopyeo-tang (BPT) is composed of six medicinal herbs ( L., (Gaertn.) DC., C.A.Mey., L.f., Schischkin, and (Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7- -glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation of compounds in both analyses was performed on a C reversed-phase column using the gradient elution of water-acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC-MS/MS analysis. As a result of analyzing the two methods, HPLC-PDA and UPLC-MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99-106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01-4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules29051171