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Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography-Photodiode Array Detector and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
Bopyeo-tang (BPT) is composed of six medicinal herbs ( L., (Gaertn.) DC., C.A.Mey., L.f., Schischkin, and (Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydro...
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| Published in: | Molecules (Basel, Switzerland) Switzerland), 2024-03, Vol.29 (5), p.1171 |
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| creator | Seo, Chang-Seob |
| description | Bopyeo-tang (BPT) is composed of six medicinal herbs (
L.,
(Gaertn.) DC.,
C.A.Mey.,
L.f.,
Schischkin, and
(Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-
-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation of compounds in both analyses was performed on a C
reversed-phase column using the gradient elution of water-acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC-MS/MS analysis. As a result of analyzing the two methods, HPLC-PDA and UPLC-MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99-106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01-4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT. |
| doi_str_mv | 10.3390/molecules29051171 |
| format | article |
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L.,
(Gaertn.) DC.,
C.A.Mey.,
L.f.,
Schischkin, and
(Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-
-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation of compounds in both analyses was performed on a C
reversed-phase column using the gradient elution of water-acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC-MS/MS analysis. As a result of analyzing the two methods, HPLC-PDA and UPLC-MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99-106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01-4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules29051171</identifier><identifier>PMID: 38474683</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acetonitrile ; Acids ; Bopyeo-tang ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Chromatography, Liquid ; Detectors ; Drugs, Chinese Herbal - chemistry ; Herbal medicine ; High performance liquid chromatography ; HPLC–PDA ; Mass spectrometry ; Medical research ; Medicine ; Medicine, Experimental ; Pulmonary fibrosis ; Quantitative analysis ; Republic of Korea ; Scientific imaging ; simultaneous quantification ; Tandem Mass Spectrometry - methods ; traditional Korean medicine ; UPLC–MS/MS</subject><ispartof>Molecules (Basel, Switzerland), 2024-03, Vol.29 (5), p.1171</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the author. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c561t-686e06ad752180842bf2bbaa9274ed936137f3fa99a4f4dbc6ffa0405eab64213</citedby><cites>FETCH-LOGICAL-c561t-686e06ad752180842bf2bbaa9274ed936137f3fa99a4f4dbc6ffa0405eab64213</cites><orcidid>0000-0002-8156-446X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2955877888/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2955877888?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38474683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seo, Chang-Seob</creatorcontrib><title>Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography-Photodiode Array Detector and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry</title><title>Molecules (Basel, Switzerland)</title><addtitle>Molecules</addtitle><description>Bopyeo-tang (BPT) is composed of six medicinal herbs (
L.,
(Gaertn.) DC.,
C.A.Mey.,
L.f.,
Schischkin, and
(Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-
-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation of compounds in both analyses was performed on a C
reversed-phase column using the gradient elution of water-acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC-MS/MS analysis. As a result of analyzing the two methods, HPLC-PDA and UPLC-MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99-106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01-4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT.</description><subject>Acetonitrile</subject><subject>Acids</subject><subject>Bopyeo-tang</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Liquid</subject><subject>Detectors</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Herbal medicine</subject><subject>High performance liquid chromatography</subject><subject>HPLC–PDA</subject><subject>Mass spectrometry</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine, Experimental</subject><subject>Pulmonary fibrosis</subject><subject>Quantitative analysis</subject><subject>Republic of Korea</subject><subject>Scientific imaging</subject><subject>simultaneous quantification</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>traditional Korean medicine</subject><subject>UPLC–MS/MS</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkstu1DAUhiMEoqXwAGyQJTYsmmLHjpOs0DBcWtFCUWfW0YlznHGV2FPHQZqH5h3wdErVARbIC1vH__8dnUuSvGT0hPOKvh1cj2rqccwqmjNWsEfJIRMZTTkV1eMH74Pk2TheU5oxwfKnyQEvRSFkyQ-Tn1dmmPoAFt00ku8T2GC0URCMs8Rp8tVYJAvwHQYyd8PaTbYdibFk4aE1WxX05IvzCJZcYGtU1B-T9269QZcuwHbHZDka25FT063SS_Ta-QGsQnJubibTkvnKuwGC6zysV5v0cuWCa41rkcy8hw35gAFVcJ6AbcmyDx7-gxITtziQCxhHcrWO_viLwW-eJ0809CO-uLuPkuWnj4v5aXr-7fPZfHaeqlyykMpSIpXQFnnGSlqKrNFZ0wBUWSGwrbhkvNBcQ1WB0KJtlNQaqKA5QiNFxvhRcrbjtg6u67U3A_hN7cDUtwHnuxp8MKrHupGNUBnVjZBSyEyAqBQVUilkmVC8iKx3O9Z6agZsFdrYg34Puv9jzaru3I-a0YqLgueR8OaO4N3NhGOoBzMq7Pvd1OusymUsWbIySl__Ib12k48zvlXlZVGU5QNVB7ECY7WLidUWWs-KUgpeZuW2CSf_UMUTR2OUs6hNjO8Z2M6gvBtHj_q-SEbr7cLXfy189Lx62J17x-8N578Av-gDlQ</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Seo, Chang-Seob</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-8156-446X</orcidid></search><sort><creationdate>20240301</creationdate><title>Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography-Photodiode Array Detector and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry</title><author>Seo, Chang-Seob</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c561t-686e06ad752180842bf2bbaa9274ed936137f3fa99a4f4dbc6ffa0405eab64213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetonitrile</topic><topic>Acids</topic><topic>Bopyeo-tang</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Liquid</topic><topic>Detectors</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>Herbal medicine</topic><topic>High performance liquid chromatography</topic><topic>HPLC–PDA</topic><topic>Mass spectrometry</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine, Experimental</topic><topic>Pulmonary fibrosis</topic><topic>Quantitative analysis</topic><topic>Republic of Korea</topic><topic>Scientific imaging</topic><topic>simultaneous quantification</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>traditional Korean medicine</topic><topic>UPLC–MS/MS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seo, Chang-Seob</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals (DOAJ)</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seo, Chang-Seob</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography-Photodiode Array Detector and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><addtitle>Molecules</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>29</volume><issue>5</issue><spage>1171</spage><pages>1171-</pages><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>Bopyeo-tang (BPT) is composed of six medicinal herbs (
L.,
(Gaertn.) DC.,
C.A.Mey.,
L.f.,
Schischkin, and
(Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-
-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation of compounds in both analyses was performed on a C
reversed-phase column using the gradient elution of water-acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC-MS/MS analysis. As a result of analyzing the two methods, HPLC-PDA and UPLC-MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99-106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01-4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38474683</pmid><doi>10.3390/molecules29051171</doi><orcidid>https://orcid.org/0000-0002-8156-446X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | Acetonitrile Acids Bopyeo-tang Chromatography Chromatography, High Pressure Liquid - methods Chromatography, Liquid Detectors Drugs, Chinese Herbal - chemistry Herbal medicine High performance liquid chromatography HPLC–PDA Mass spectrometry Medical research Medicine Medicine, Experimental Pulmonary fibrosis Quantitative analysis Republic of Korea Scientific imaging simultaneous quantification Tandem Mass Spectrometry - methods traditional Korean medicine UPLC–MS/MS |
| title | Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography-Photodiode Array Detector and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
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